Pulse-labeled RNA in sea urchin embryos is largely confined to the nucleus even after removal of membranes with detergents. Various reconstruction controls eliminated extensive cytoplasmic contamination of the nuclear preparations although a small amount of contamination by ribosomes or polyribosomes could...
Mouse (erythroleukemia, TSA8, and FM3A) cells and human (HeLa and HL-60) cells were pulse-labeled with [ 3H]thymidine and covalently closed circular DNA in the extrachromosomal fraction was analyzed by fluorography following polyacrylamide gel electrophoresis. Two discrete bands for mouse and at ...
【题目】1.Pathway of Atoms in FermentationA "pulse-chase" experiment using 14C-labeledcarbon sources is carried out on a yeast extract maintained under strictly anaerobic conditions to produce ethanol.T he experiment consistsof incubating a small amount of 14C -labeledsubstrate (the pulse)with the...
A suite of multiple quantum (MQ) HCN-based pulse sequences has been developed for the purpose of collecting dipolar coupling data in labeled nucleic acids. All the pulse sequences are based on the robust MQ-HCN experiment which has been utilized for assignment purposes in labeled nucleic acids...
Pulse-labeled polysomal RNA was characterized at T4 in naturally permeable cells of strain SK-1 and impermeable cells which required manipulation of the medium pH to facilitate uptake. Transfer ribonucleic acid (RNA), poly A-containing RNA and ribosomal RNA were synthesized in both cultures during ...
The distribution of pulse-labeled polypeptide chains in rat liver deoxycholate-soluble protein has been obtained by sodium dodecyl sulfate-gel electrophoresis and used to compute a theoretical curve for determination of synthesis time of a 50 000 mol. wt. polypeptide chain ( t c 50). Values of ...
Uracil incorporation: a source of pulse-labeled DNA fragments in the replication of the Escherichia coli chro- mosome. Proc. Natl. Acad. Sci. USA 75:233-237.Tye, B. K., J. Chien, I. R. Lehman, B. K. Duncan, and H. R. Warner. 1978. Uracil incorporation: a source of pulse-...
& PETIT C. (1993) The normal sequence of phenotypic transitions in one cohort of BrdUrd-pulse labeled thymocytes: correlation with T cell receptor expression. J Immunol 151, 4574.Lucas B, Vasseur F, and Penit C. Normal sequence of phenotypic transitions in one cohort of 5...
Pump J, Conrad R (2014) Rice biomass production and carbon cycling in 13CO2 pulse-labeled microcosms with different soils under submerged conditions. Plant Soil 384:213-229Pump, J., and Conrad, R. (2014) Rice biomass production and carbon cycling in 13CO2 pulse- labeled microcosms with ...