The homemade pull-down assay represents a collection of reagents from multiple commercial vendors that cannot be validated together as a functional assembly except by extensive assay development by the researcher, and troubleshooting this combination of reage...
The homemade pull-down assay represents a collection of reagents from multiple commercial vendors that cannot be validated together as a functional assembly except by extensive assay development by the researcher, and troubleshooting this combination of reagents ...
Pull-Down System are provided in this section. Please refer to Section IV for specific protocol details. Controls Appropriate controls should be performed during each phase of the MagneGST? Pull-Down assay. In Phase 1, it is important to verify that the prey protein is expressed in the TNT?
3.A. Controls Appropriate controls should be performed during each phase of the MagneGST™ Pull-Down assay. In Phase 1, it is important to verify that the prey protein is expressed in the TNT® reaction. Load 1–5% of the TNT® reaction product onto an SDS-PAGE gel and verify the...
Profacgen provides professional service for protein pull down assay. We have technical service group and most advanced instruments to perform the entire procedure with high-efficiency and high-quality. Background Pull down assay is an in vitro method to detect protein-protein interaction. The ...
For users who would like to subject the pull-down products for Mass Spectrometry as further application, at least 10 mg proteins for each pull-down assay should be prepared to ensure that at least 1 mg proteins should be pulled down and sent for mass spectrometry analysis. CRITICAL: It is ...
The basic pull-down assay is an in vitro technique that consists of a polyhistidine-tagged bait protein (the researcher’s protein of interest) that can be used to identify putative binding partner(s) (the prey). The bait protein, purified from an appropriate expression system (e.g., ...
Two ml of SNAP-Capture Pull Down Resin is sufficient to perform 25 pull down assays using 80 µl of resin suspension per assay. The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on ...
The control system for the pull-down assay uses 3? untranslated-region androgen receptor (AR) RNA, poly(A)25 RNA and mammalian cell lysate. The proximal 3? untranslated region (UTR) of AR RNA contains UC-rich regions for HuR and Poly(C) Binding Proteins (CP1 and CP2). These RNA-...
Figure 2.Results from BK035 Rac1 activation assay. Activated Rac1 was precipitated and detected in a Western blot using kit BK035. The first lane shows a 50 ng recombinant His-tagged Rac1 standard (Recombinant His-Rac1). The following lanes shows the pull-down of inactive, GDP-loaded Rac...