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In addition, the PNX system may be especially complex in fish, where an additional receptor is likely present in some species. The purpose of this study was to assess hypothalamic and ovarian transcriptomes after PNX-14 administration in female vitellogenic green-spotted puffer (Dichotomyctere ...
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The puffer fish were anaesthetized by qualified staff in MS222 and the internal organs and blood were removed prior to all analyses. Measurement of quality traits The methods for measuring the Quality traits of puffer fish muscle The quality traits of the puffer fish muscle in all three groups ...
The juveniles of hatchery-reared non-toxic tiger puffer (mean body weight (BW): 1.3 g) obtained from Nagasaki Fishery Public Corporation, Sasebo, Nagasaki, Japan, were anesthetized by immersion in 200 ppm of MS222. The brain with pituitary was excised, fixed with Bouin's fluid for 24...
MS/MS fragmentation pattern of the peak (Figure 3B) was essentially similar to that of the standard TTX. AnhydroTTX and deoxyTTX were also detected in the extract (data not shown). Extract of muscle (the edible part) of the same T. nigroviridis also contained TTX, the MS/MS ...
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For IP administration of AVT, one fish was placed in a small tank under anesthesia with 0.05 % MS-222, then 1 μL g−1 BW of each test solution was injected intraperitoneally using a small 250-μL Hamilton syringe. The fish in the experimental group were injected with 1 μL g...