材料和方法部分: pUC57-sgRNA expression vector was a gift from Xingxu Huang (Addgene plasmid # 51132 ; http://n2t.net/addgene:51132 ; RRID:Addgene_51132) 参考文献部分: Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects. Shen B, Zhang W, Zhang J, Zhou ...