表1 pMD18-T载体与pUC19-HB-T载体连接PCR产物效率比较Table 1 Ligation efficiency test of pMD18-T vector and pUC19-HB-T vector with PCR product注:a表示线性化载体在Solution I作用下自身环化试验。Note:“a”represents that linearized vectors were made an own-cyclization test under the effect of Sol...
[结果]经rrA克隆验证,制备的pUC19一HB—T载体对PCR 产物的克隆率达到95%以上.[结论]构建的pUC19一HB— T载体可用于PCR产物的克隆,测序及后续分子生物学操作. 关键词T载体;XcmI限制性内切酶;TA克隆 中图分类号S132文献标识码A文章编号0517—6611(2011)17—10182—03 ConstructionofApUC19.TVectorBasedonXcmI SUN...