Admiral Edward Vernon had a long and distinguished naval career, first promoted to captain in 1706 and going on to serve as a vice-admiral during the War of Jenkin’s Ear between Britain and Spain (1739-48) – during which he famously captured the Spanish colonial possession of Porto Bello ...
Royal Oak, Hampton White Swan, Twickenham (3 nominations). SUTTON The Hope, Carshalton (3 nominations). "Shortlisted for CAMRA local pub of the year." Wallington Arms, Wallington WALTHAM FOREST The Bell, Walthamstow The Coppermill, Walthamstow Leyton Technical, Leyton Ye Olde Rose and Crown, Wa...
Sequences were read on an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems Inc, Waltham, Massachusetts). Primers, PCR, and sequencing conditions are detailed in the Supplemental Information, Appendix S1: Tables S1, S2. All previously unpublished sequences have been deposited in GenBank; DNA ...
(2014). DNA concentration was quantified using a Qubit 4 fluorometer with the dsDNA HS assay kit (Thermo Fisher Scientific, Waltham, MA, USA). A subset of 15 samples was randomly chosen for analysis on the Agilent 4200 TapeStation (Agilent Technologies, Santa Clara, CA, USA) using a ...
Homogeneous time-resolved fluorescence energy transfer (HTRF) assays were performed using the Lance Ultra cAMP kit (PerkinElmer, Waltham, MA, USA), based on the competitive displacement of an europium chelate-labelled cAMP tracer bound to a specific antibody conjugated to acceptor beads. We first es...