DEBOER AND YONGLI LI Department of Pediatrics, University of Virginia, Charlottesville, Virginia 22908 ABSTRACT: In boys, inflammatory bowel disease often results in delayed puberty associated with decreased bone mineral density and decreased linear growth. Our goal was to investigate whether puber- tal...
With such major physical, emotional and psychological development at this stage in their lives, boys and girls need support and information which the school nurse is well placed to provide. Based on extensive experience of giving talks and working with pupils of this age group, the authors ...
We did immunohistochemical staining with PLAP, anti-OCT3/4, anti-c-Kit and anti-D2-40 in 1521 consecutive testicular biopsies from 1134 boys aged 1 month to 16½ years operated for UDT. OCT3/4 and D2-40-positive germ cells, probably gonocytes, were found throughout the first ...
Genomic DNA was extracted from peripheral blood leucocytes using the QIAamp DNA blood mini-kit (Qiagen) following the manufacturer’s instructions. As previously reported, exons 1–5 of the SRD5A2 gene (25) and exons 2–7 of the NR5A1 gene (26) were amplified by PCR, and direct sequencin...
Biotin-labeled cRNA was then synthesized from the double-stranded cDNA by in vitro transcription using a BioArray HighYield RNA Transcript Labelling Kit (Affymetrix). The cRNA was then purified using an RNeasy Mini kit (QIAGEN), fragmented, and hybridized to murine genome U74A chips for 16 h....
Biotin-labeled cRNA was then synthesized from the double-stranded cDNA by in vitro transcription using a BioArray HighYield RNA Transcript Labelling Kit (Affymetrix). The cRNA was then purified using an RNeasy Mini kit (QIAGEN), fragmented, and hybridized to murine genome U74A chips for 16 h....
Plasma hormonal levels were measured by the enzymatic immu- noassay kit as described below, and the area under the curve for each hormone and the ratio between the two hormones were calculated. Another set of animals (n ¼ 6 per group) was used to measure the targets of the attacks ...
The impact of aromatase inhibitor therapy on bone turnover, cortical bone growth and vertebral morphology in peripubertal boys with idiopathic short stature Introduction: The role of estrogen in the regulation of bone mass accrual, cortical bone growth, and bone turnover in males during puberty has...
Subjects were considered normal-weight or obese when their projected BMI value for the age of 18 y was lower than 25 kg/ m2 or between 30 and 35 kg/m2, respectively (34). Of the 117 origi- nally recruited boys, 23 were obese, representing a ratio 19.66% that approximates the ...
We recently defined precocious puberty in the Chinese population, setting the onset before 7.5 and 9 years of age for girls and boys, respectively [4]. Additionally, childhood overweight and obesity are associated with an increased risk of precocious puberty in China [4]. Similarly, our animal ...