Serum PTH was measured with an intact molecule immunochemiluminometric assay and 1,25(OH)2D was measured with radioimmunoassay after HPLC separation. Hypertensive men had PTH levels that were 36% higher than normotensive men (5.3 +/- 2.9 v 3.9 +/- 0.8 pmol/L, P = .005). When blood ...
Residues in the (17-31) domain of PTH(1-31)NH2 were individually replaced by alanine, and effects on binding to the intact PTHR (FIG. 1A and FIG. 1B) and to PTHR-delNt (FIG. 1C and FIG. 1D) were assessed by competition methods. Assays in FIG. 1A and FIG. 1B used stably ...