B cells (mouse) were lysated in RIPA buffer with protein and phosphatase inhibitors. 10 µg of protein per lane were loaded in a 10% PAGE-SDS gel. Proteins were transferred to a PVFD membrane (Turbo Blot system) and incubated overnight with 1:1000 of anti-TLR4, followed by anti-rabb...
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Purified recombinant green fluorescent proteins EGFP, TurboGFP and mNeonGreen were used as negative controls. Pan-RFP (pabr1) antibody was applied at 1:1000 dilution o/n at +4°C. Secondary antibody: goat anti-rabbit HRP. Note: Red fluorescent proteins often demonstrate multiple bands on ...
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