磷酸酶张力蛋白同源物基因肝纤维化肝星状细胞 PTENhepatic fibrosishepatic stellate cellObjective To explore the role of expression of PTEN protein in fibrotic liver tissue during pathological process of hepatic fibrosis in rats.
磷酸酶及张力蛋白同源体绿色荧光蛋白高尔基体为了明确pten敲除小鼠中转录上调基因pdd87编码蛋白在细胞中的定位,构建PDD87与绿色荧光蛋白(GFP)的融合蛋白表达载体pEGFP-C1-pdd87,利用Lipofectamine2000转染该载体于体外培养的NIH3T3细胞中,采用高尔基体特异探针BODIPYTRC5-Ceramide染色显示高尔基体,激光共聚焦显微镜下观察到PDD...