Do not aliquot the antibody. Protocol Specificity / Sensitivity PTBP1 (E5O2S) Rabbit mAb recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced ...
Antibody 克隆号 7 抗原 Recombinant human PTB protein. 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Protein A 保存液 PBS, pH 7.4 内含物 0.1% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) RRID AB_2533083 靶标信...
Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. We use
Antibody 克隆号 9M6A8 抗原 A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (P26599) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.22 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 50% glycerol, 0.05% BSA ...
IRDye 800CW goat anti-mouse IgG antibody LI-COR Biosciences Cat# 926-32210; RRID:AB_621842 Chicken anti-rat Alexa Fluor 488 ThermoFisher Cat# A21470; RRID: AB_2535873 Goat anti-rabbit IgG Alexa Fluor 546 ThermoFisher Cat# A11010; RRID AB_2534077 Goat anti-mouse IgG Alexa Fluor 546 Ther...
AIn vitro RNA pull-down assay in LN229 cell line. Proteins were subjected to western blot analysis with anti-PTBP1 antibody after pull-down.BAnalysis of PTBP1 CLIP-seq data from GSE19323 and GSE114720, the blue lane shows PTBP1 CLIP peaks for exon 30 and adjacent area of introns of IT...
The immune complexes were centrifuged and washed, and proteins were analyzed by western blotting with an anti-ubiquitin antibody (3936, Cell Signaling Technology) following the manufacturer’s instructions. FISH (fluorescence in situ hybridization) The FAM (fluorescein amidite)-labeled circSCP2 RNA ...
mouse anti-SRSF3 (clone 7B4, invitrogen, USA), monoclonal mouse anti-PTBP1 (Santa Cruz Biotechnology, USA), monoclonal rabbit anti-PTBP2 (Epitomics, USA), monoclonal rabbit anti-UPF1 (Cell Signaling Technology, USA), or horseradish peroxidase-labeled mouse anti-β-actin antibody (Sigma-...
After blocking, membranes were incubated overnight at 4°C in blocking solution containing primary antibody. Blots were washed and incubated with the appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies for 1 hr at room temperature. Proteins were visualized and quantified using an ...
The immune complexes were centrifuged and washed, and proteins were analyzed by western blotting with an anti-ubiquitin antibody (3936, Cell Signaling Technology) following the manufacturer's instructions. FISH (fluorescence in situ hybridization) The FAM (fluorescein amidite)-labeled circSCP2 RNA ...