Do not aliquot the antibody. Protocol Specificity / Sensitivity PTBP1 (E5O2S) Rabbit mAb recognizes endogenous levels of total PTBP1 protein. This antibody does not cross-react with PTBP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced ...
Antibody 克隆号 7 抗原 Recombinant human PTB protein. 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Protein A 保存液 PBS, pH 7.4 内含物 0.1% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) RRID AB_2533083 靶标信...
Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. We use
Antibody 克隆号 ARC0783 抗原 A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (P26599) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.22 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 50% glycerol, 0.05% BSA ...
After fixation with 4% paraformaldehyde, the cells were blocked with 5% BSA for 30 min at room temperature and incubated with anti-HA monoclonal antibody (1:1000, Cell Signaling Technology, USA) overnight at 4 °C, then washed three times and surface-bound monoclonal antibody detected ...
The immune complexes were centrifuged and washed, and proteins were analyzed by western blotting with an anti-ubiquitin antibody (3936, Cell Signaling Technology) following the manufacturer’s instructions. FISH (fluorescence in situ hybridization) The FAM (fluorescein amidite)-labeled circSCP2 RNA ...
The immune complexes were centrifuged and washed, and proteins were analyzed by western blotting with an anti-ubiquitin antibody (3936, Cell Signaling Technology) following the manufacturer’s instructions. FISH (fluorescence in situ hybridization) The FAM (fluorescein amidite)-labeled circSCP2 RNA ...
AIn vitro RNA pull-down assay in LN229 cell line. Proteins were subjected to western blot analysis with anti-PTBP1 antibody after pull-down.BAnalysis of PTBP1 CLIP-seq data from GSE19323 and GSE114720, the blue lane shows PTBP1 CLIP peaks for exon 30 and adjacent area of introns of IT...
The monoclonal antibody mouse anti-human PTBP1 was purchased from Abway (1:200 dilution) and the chromogenic kit was purchased from DAKO (K5007, 1:100 dilution). PBS buffer (Na2HPO4 8.1 mmol/L, KH2PO4 4.5 mmol/L, NaCl 137 mmol/L, KCl 2.7 mmol/L, pH 7.4) was shaken and washed. ...
Then the cell lysates were incubated with the antibody-bead complex at 4°C overnight. The beads were washed by wash buffer six times before the RNA was extracted and precipitated. The quality and abundance of hsa_circ_0005358 or CDCP1 mRNA were detected by qRT-PCR. RNA-sequencing analysis...