Polypyrimidine tract binding protein 1 (PTBP1) has been found to play an important role in the occurrence and development of various tumors. At present, the role of PTBP1 in gastric cancer (GC) is still unknown and worthy of further investigation. We use
Antibody 抗原 A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_1143681) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 0.64 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 50% glycerol 内含物 0.05% ProClin 300 ...
Antibody 抗原 Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human PTBP1 (NP_1143681) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 5.31 mg/mL 纯化类型 Affinity Chromatography 保存液 PBS, pH 7.3, with 50% glycerol 内含物 0.02% sodiu...
AIn vitro RNA pull-down assay in LN229 cell line. Proteins were subjected to western blot analysis with anti-PTBP1 antibody after pull-down.BAnalysis of PTBP1 CLIP-seq data from GSE19323 and GSE114720, the blue lane shows PTBP1 CLIP peaks for exon 30 and adjacent area of introns of IT...
mouse anti-SRSF3 (clone 7B4, invitrogen, USA), monoclonal mouse anti-PTBP1 (Santa Cruz Biotechnology, USA), monoclonal rabbit anti-PTBP2 (Epitomics, USA), monoclonal rabbit anti-UPF1 (Cell Signaling Technology, USA), or horseradish peroxidase-labeled mouse anti-β-actin antibody (Sigma-...
Magnetic beads coated with protein A/G were treated with an anti-Lin28A antibody from Proteintech, USA, or a negative control IgG antibody. Subsequently, antibodies were incubated with BLca cells. The coprecipitated RNA was then purified and enriched by qRT-PCR assay. The primer sequences can ...
To further verify the interaction between PTBP1 andANCR, we performed an RNA immunoprecipitation (RIP) assay in hAMSCs. As shown in Fig.7c, PTBP1 antibody (anti-PTBP1) significantly precipitatedANCRas well asID2compared with an anti-IgG control. Using siRNA silencing to decrease the expression...
Magnetic beads were washed twice with the immunoprecipitation lysis/wash buffer and once with water. After elution of antigen/antibody complexes, the WB with anti-Ubiquitin (Invitrogen, cat. no.: 701339) was performed. PTBP1 and c-Myc protein levels were also determined in whole-cell lysates (...
Antibody 克隆号 7 抗原 Recombinant human PTB protein. 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Protein A 保存液 PBS, pH 7.4 内含物 0.1% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) RRID AB_2533083 靶标信...
Antibody 克隆号 7 抗原 Recombinant human PTB protein. 偶联物 Unconjugated 形式 Liquid 浓度 0.5 mg/mL 纯化类型 Protein A 保存液 PBS, pH 7.4 内含物 0.1% sodium azide 保存条件 -20°C 运输条件 Ambient (domestic); Wet ice (international) ...