产品名称: psiCHECK(TM)-2 Vector .0 订购此产品 供应商: Promega 规格: 20ug 目录价: 3395 库存状态: 二周到货 CAS编号: 应用范围: 生化试验 种属来源: 相关信息: 说明 psiCHECK™ -1 和psiCHECK™ -2 载体用于对RNA干扰(RNAi)的早期优化提供快速的定量方法。此载体可监测与报告基因融合的目的基...
vector control. Because miRNAs affect translation and not transcriptional activation, both the miRNA reporter and internal control can be expressed from the same plasmid without the danger of the miRNA reporter regulation affecting the control.Here we demonstrate the use of the psiCHECK™-2 Vector ...
psiCHECK-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. In both vectors, Renilla luciferase is used as a primary reporter gene...
psicheck2 Vector Information 载体名称: psicheck2 质粒类型: 哺乳动物细胞表达系统载体,双荧光素酶报告质粒 表达水平: 高拷贝 启动子: T7 promoter,SV40 promoter,HSV TK promoter 克隆方法: 多克隆位点,限制性内切酶 克隆位点: MCS 载体大小: 6273bp 5' 测序引物及序列: Psicheck2.F:TGAAGGGCCTCCACTTCAGC 3'...
psiCHECK-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene. In both vectors, Renilla luciferase is used as a primary reporter gene...
-1 Vector psiCHECK?-2 Vector Storage Conditions: Store the psiCHECK?-1 and psiCHECK?-2 Vectors at –20°C. Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA Toll Free in USA 800-356-9526 · Phone 608-274-4330 · Fax 608-277-2516 · www.promega.com Part# ...
目录价: ￥0.00 货号:C8021 名称:psiCHECK™-2 Vector 品牌:Promega 规格:20µg 购买数量: 立即购买 产品信息
We demonstrated use of the psiCHECKTM-2 Vector as abiosensor for miRNA activity and as a screening tool formiRNA targets. By cloning the inverted complement of miR-21 into the psiCHECKTM-2 Vector, we were able to look at relative differences in miR-21 activity between cell lines. By ...
A.RestrictionEnzymeSitesforthepsiCHECK™-1Vector...11 B.RestrictionEnzymeSitesforthepsiCHECK™-2Vector...13 6.siCHECK™VectorBackbonesandComponents...15 7.References...16 8.RelatedProducts...
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