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Cells migrating into the scratched filed were recorded by phase contrast microscopy (DM400B, Leica Corporation, Germany) at 24 h after the scratch. Photographs of cells migrating into the scratched filed were taken for analysis of migration ability. Trans-well migration and invasion assay We ...
pDONR221 using the BP clonase (Invitrogen), amplified and further shuttled into the LUMIER prey vector (FireV5DM). Human full-length cDNA clones of SNAP25, PSD95, and CTTN were available as entry clones and shuttled into the respective LUMIER bait (PAReniDM) and prey (FireV5DM) vectors....
The initial association rates (α values) were obtained by linear regression in the first 20 s of peptide binding, when almost all adsorbed lipid vesicles on the chip surface were free and dissociation rate constant was insignificant (kon>>>koff). The approximate association rates were calculate...
00 Motor Tuning Condition [0] Free [1] Lock Main Menu Page[3] Auto Tuning 3 A3-1 "Motor Tuning: 执行 (参考7.3章) 转到"7.4.3章速度或频率指令及数字量输入设定" 说明 设定V/F速度控制 电机未安装制动或自学习时制动可以脱开 (注意:需要电机处于空载状态) 电机安装制动自学习时制动无法脱开时 ③...