产品名称:PSA-ACT Complex 产品型号: 产品展商:其它品牌 产品文档:无相关文档 简单介绍 PSA-ACT Complex PSA-ACT Complex的详细介绍 Product NamePSA-ACT Complex SourceHuman Semen & Serum Catalogue Number498-11 Purity>96% (SDS-PAGE) FormFrozen liquid ...
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Product NamePSA-ACT Complex SourceHuman Semen & Serum Catalogue Number498-11 Purity>96% (SDS-PAGE) FormLiquid Protein0.5 - 3.0 mg/mL (A280, E0.1%= 1.0) Formulation0.2 μm filtered solution in 0.05 M sodium phosphate pH 7.5 with 0.15 M sodium chloride and 0.09% sodium azide (Cat. No:...
It is responsible for the proteolysis of the gel-forming proteins in human semen. Two major extracellular protease inhibitors, alpha-1-antichymotrypsin (ACT) and alpha-2-macroglobulin (AMG) may inactivate PSA escaping from the prostate. The predominant immunodetected form of PSA in serum is ...
Analysis of the PSA-ACT complex To analyse the effect of the rs17632542 KLK3 SNP in complexing of PSA variants with ACT (predominant PSA inhibitor in serum), 0.1 µM recombinant mature PSA proteins (generated in Pichia pastoris), Wt, Thr163 and Ala195 were incubated with ACT (0.5...
PSA is mainly responsible for gel dissolution in freshly ejaculated semen by proteolysis of the major gel forming proteins. The major part (70-90 %) of PSA in serum is complexed to alfa1-antichymotrypsin (ACT). Total PSA (free+ACT-complex) is increased in both benign prostate hyperplasia ...
See supplementary results for the production, purification, isotyping, calculation of the apparent Kd (Supplementary TableS1), biotinylation, serum competition (Supplementary Fig.S1), SDS-PAGE and Western blots, reactivity of anti-PSA mAbs on immunoblots towards fPSA and PSA-α1ACT complex (Supplemen...
A few per cent of PSA in semen is complexed to the protein C inhibitor. PSA complexed to 伪1-antichymotrypsin (ACT) constitutes the predominant molecular form of serum PSA, although complex formation is slow between the purified proteins in vitro. PSA also forms stable complexes with 伪2-...