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A portion of the PS1 holoprotein, an ∼45 kDa protein, is cleaved in the endoplasmic reticulum (ER) to create a two-chain form (Zhang et al., 1998). Many known PS1 functions, but not all, involve the cleaved form of PS1 as the catalytic subunit of the gamma (γ)-secretase enzyme...
Moreover, overexpression of DISC1 in the hippocampus with an adeno-associated virus reduced the levels of BACE1, soluble Aβ40/42, amyloid plaque density, and rescued cognitive deficits of APP/PS1 transgenic mice. These results indicate that DISC1 attenuates Aβ generation and cognitive deficits of...
and blood was collected from the right ventricle by using a 25-gauge needle and stored in buffered sodium citrate blood collection tubes (catalog number 366393; BD Vacutainer) on ice. After the blood collection, mice were perfused by using cold for Hank’s balanced salt solution (HBSS) (Gibco...
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For western blot, 30 μl of each sample was loaded onto 4-20 % SDS-PAGE gels, electrophoresed, transferred to PVDF membrane and probed with mouse anti-brevican (BD Biosciences 610895, San Jose, CA). Mice and surgery Mouse protocols used in these experiments were approved by the Institute...
Disrupted-in-Schizophrenia-1 (DISC1) is a genetic risk factor for a wide range of major mental disorders, including schizophrenia, major depression, and bipolar disorders. Recent reports suggest a potential role of DISC1 in the pathogenesis of Alzheimer
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