Full size image We next performed an ELISA assay to detect Aβ42 in brain tissue supernatants collected from both experimental groups to quantify the level of amyloid protein in the brain. At 12 weeks of ethanol exposure, Aβ42 levels were markedly reduced in the cortex and hippocampus of eth...
Proteins were transferred to nitrocellulose membranes (Bio-rad, Hercules CA), and blocked for 1 hour at room temperature in blocking buffer which was a 5% non-fat milk (Labscientific, inc, Livingston, NJ) and tris buffered saline containing 0.1% tween-20 solution (Boston Bioproducts, Ashland...
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4-month old APP/PS1 mice were randomly put into two groups and fed with either Probio-M8 or saline water for 45 days. Fecal samples of mice were collected at the beginning and the end of the treatment period to determine the composition of the gut microbiota via 16S ribosomal RNA sequencin...
Full size image Fig. 7 Influence of FMT treatment on inflammation-related signaling pathways in the brain of APP/PS1 mice. (A) Representative images of hippocampal TLR4, NF-κB, COX-2, MyD88, IL-6, IL-1β and TNF-α in Western blot experiments. (B-H) Quantification of TLR4 (F =...
Full size image Immunohistochemical staining for the Na+/K+ ATPase αIII subunit was performed using saggital sections from non-transgenic and APP+PS1 mice. Specific staining was observed throughout the forebrain. The specificity of the Na+/K+ ATPase α subunit antibody used for immunochemistry ...
Full size image Normalized GFAP coverage to cell-associated amyloid is increased in the DG but not CA region in 4 months APP/PS1 mice (DG: Mann-WhitneyU = 5.0,p = 0.0221, Fig.5b, CA:t(11) = 1.532,p = 0.1538, not shown). In the 10 months APP/PS1 mice cl...
The membranes were blocked for 30 min using 5% non-fat dry milk in Tris-buffered saline (TBS) containing 0.5% Tween-20 (TBST), then probed with specific primary antibodies against NMDAR1 (Abcam, ab109182), GluR1 (Abcam, ab31232), GluR2 (Abcam, ab206293), GABAB1R (Abcam, ab55051)...
Full size image On the whole, the behavioral, histopathological, and molecular data above suggest that the APP/PS1 mice on the high fat diet in this study showed a similar exacerbation of Alzheimer’s-related effects as in previous studies16,17,22. We next sought to determine the role of ...
Full size image Voluntary running exercise increased hippocampal volume and reduced amyloid plaque deposition in APP/PS1 mice There were significant differences in the volumes of DG (F = 6.490,p = 0.004) and CA1 (F = 7.558,p = 0.002) regions among the four groups, while ...