PRRSV基因组的长度约为15,000个nt,尾部包含有ORF1a在内的至少11个ORFs(图2)。其中,ORF1a和ORF1b编码16种非结构蛋白(Nsp)。ORF2TF是在PRRSV的Nsp2区域中发现的一种新的ORF,产生另外两个Nsp:Nsp2N和Nsp2TF。其余的ORF2a、2b、3、4、5...
这些策略包括nsp1β、nsp4、nsp2TF和nsp2N的修饰、建立嵌合病毒、密码子反优化等。然而文献综述表明，特定PRRSV毒株的毒力至少与上述蛋白加上nsp2、nsp9、nsp10、GP2、GP3、GP5和M有关。总之，毒力很可能涉及多个基因，与复制、炎症反应的诱导或抑制，特异性免疫的产生和凋亡机制有关。目前，很难预见某一策略可...
Nonstructural proteins nsp2TF and nsp2N of porcine reproductive and respiratory syndrome virus (PRRSV) play important roles in suppressing host innate immune responsesY.ShangP.ShyuD.CarrilloC.Naraghi-AraniJaingCrystal J.VIROLOGY -NEW YORK-
处形成 10个非结构蛋 白(nonstrueturalprotein,NSP):即 本文从 PRRSV的病毒基因组、非结构蛋 白和结构蛋 白3 NSPlct、NSP1B、NSP2～NSP6、NSP7c~、NSP715、NSP8t。最新 个方面对 PRRSV的病毒蛋 白研究进展进行综述 。 研究表明,病毒感染 6h后,10个非结构蛋 白均可检测到; 在感染早期 ,nspl~、nsp2...
Second, PRRSV nsp1 could negatively regulate IFN-β production by inhibiting the phosphorylation of IRF-3. Download: Download full-size image Fig. 5. Proposed model illustrating how PRRSV nsp1 negatively regulates IFN-β signaling pathways. Other TF: other transcription factor.?: it is unclear ...
Oleksiewicz MB, Botner A, Toft P, Normann P, Storgaard T (2001) Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. J Virol 75:3277–3290 Article PubMed CAS PubMed Central...
WK141与NVDC-NM3核苷酸同源性最高为93.5%.Nsp2,ORF3和ORF4推导aa序列比对结果表明,本研究中ZD1株的ORF3提前26个aa终止,另外WK14,WK141,WG9和HN7株在ORF3的246位存在1个aa的缺失;WK14,WK141,WG9,HN7和ZD1株PRRSV在ORF4的66位皆存在1个a...
猪繁殖与呼吸综合征病毒非结构蛋白NSP2的原核表达及其单克隆抗体的制备 将猪繁殖与呼吸综合征病毒(PRRSV)S1株截短修饰的NSP2蛋白基因(tNSP2),克隆于pGEX-6P-1载体中,转化大肠杆菌后用IPTG诱导表达。Western blot结果表明,融合表达的GST-tNS... 王海燕,姜平,杜以军,... - 《中国生物工程杂志》 被引量: 32发表...
The objective of the present study was to determine the cross-protection of Ingelvac PRRS MLV against challenge with the new lineage 1 PRRSV emerged in China in pigs. Two lineage 1 PRRSV strains (FJZ03 and FJWQ16 originated from recombination event betwe
Porcine reproductive and respiratory syndrome virus (PRRSV) is the major pathogen in the pig industry. Variability of the antigens and persistence are the biggest challenges for successful control and elimination of the disease. GP5, the major glycoprote