GP5蛋白免疫原性提供了基础数据,也为PRRS重组活裁体疫苗的研究奠定了基础。 关键词:猪生殖与呼吸综合征病毒;GP5蛋白;重组牛痘病毒 中图分类号:$852.65 文献标识码:A 文章编号:1008.0589(2009)11-0833.05 ExpressionofPRRSVGP5inrecombinantvacciniavirus TIANYe,ZHANGWei-jun,LINYan,WANGQun,YANGTao, ...
expression levelbyRT-PCR.ThePRRSV specific T lymphocyte proliferation testwereusedattheendofthe eighth weekafterimmunization.The lung、intestine、fecal fluidandserumweretakentoassaythe specificIgaorIgG against GP5withIndirectImmunofluorescent Assay.The ...
Expression of GP5-M fusion protein of porcine reproductive and respiratory syndrone virus (PRRSV) and establishment of ELISA diagnose based on the recombinant fusion protein. Sheng Wu Gong Cheng Xue Bao 21, 259-264.江云波,方六荣,肖少波. 猪繁殖与呼吸综合征病毒(PRRSV)GP5-M在大肠杆菌中的融合表达...
GP3-GP5-M蛋白表达Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of economic losses in pigs (Sus domesticus), causing severe reproductive failure in pregnant sows and boars, and respiratory problems in young pigs. This study was aimed to detect the exp...
GP5 protein obtainednamed 4A11、3D10、1E9 1H11华中农业大学2013届专业学位硕士学位论文 IV Mice were injected largequantity constructedeukaryotic expression plasmid PCAGGS HA SynORF5 Helacells expressedprotein collectedWestern blotting IFAconfirmed 1H11strains monoclonalantibody reacted specifically eukaryotically...
2.1 GP5/E蛋白PRRSV E基因长603 bp左右,编码GP5糖基化包膜蛋白(又称E蛋白),在所有结构蛋白中是变异程度最高的一种蛋白,但GP5是重要的抗原保护性蛋白,能诱导高滴度的中和性抗体,具有最强的中和能力,在保护性免疫应答中发挥重要作用。Rodrigue等研究缺失跨膜区的GP5基因在大肠杆菌中获得良好的表达,且表达产物可与PR...
Cloning and Construction of Expression Vector of GP5 Gene of PRRSV Hn-1/06 StrainPRRSVGP5基因RT-PCR表达载体[目的]克隆PRRSV Hn-1/06株GP5蛋白基因并构建其原核表达载体.[方法]根据PRRSV美洲株ATCC-VR2332的ORF5基因序列设计特异性引物,用RT-PCR方法从Hn-1/06株中扩增得到GP5蛋白基因的片段,与PTG19-T...
Meng cloned the GP5 gene into a eukaryotic expression plasmid under the control of the cytomegalovirus early promoter to prepare a DNA vaccine, which could induce the production of antibodies after immunization of piglets [41]. More and more studies showed that miRNAs could be used to treat PRRS...
However, co-expression of GP5, M and N was insufficient to release VLPs into the culture medium in EAV, suggesting that other factors (NSPs or host proteins) are required for particle formation and release (Wieringa et al., 2004). From sequence-based topology prediction (TMHMM and HMMTOP),...
Intriguingly, administering baicalein during PRRSV infection (co-treatment) and after PRRSV infection also led to a significant and time-dependent decrease in the PRRSV copy number (Figures4B2, B3), the relative expression of PRRSV’s ORF7 mRNA (Figures4C2, C3) and the virus titer (Figures4D...