substrate. Whereas the F40T, C164A, and F40T/Y42S mutants show WT-level coumarin synthase activities, the Y42F mutant shows a modest decrease in activity (Fig.2e, Supplementary Fig.11, and Supplementary Table2). These results suggest that none of the three residues are essential for the co...
Figure4(a)plots % baseline GABA:Cre for 2DJ-resolved MRS versus % baseline GABA+ for MEGAPRESS (n = 18,r2 = 0.77, p < 0.001). The corresponding BA analysis for the two techniques displayed in Fig.4(b)and, at the 5% level, normality could not be rejected for the with...
If you have any other enquiries, please leave a message. * Indicates a Required Field *Name *Email Phone *Comments: Frequently Asked Questions Question 1: How to dissolve it? Answer: S1413 Bafilomycin A1(Baf-A1) is soluble in DMSO at 6 mg/ml. Please do not use alcohols as solvent, be...
This absence probably stems from the limitation of NMR and X-ray crystallographic techniques in their ability to acquire high-resolution structures from proteins associated with membranes [40]. In fact, only 1% of structural PDB entries are membrane proteins. We nevertheless considered the possibility...
PROBES FOR ENZYME-CATALYZED PROTON-TRANSFER REACTIONSTriosephosphate isomerase1H NMR spectroscopy is a powerful analytical method for monitoring proton transfer from carbon inD2O. It has been successfully used in determination of the carbon acidity of simple functional groups[2–8,18]. This technique pro...
V-H+PPase proteins have not been crystallized, and there is no structural entry associated with them in the Protein Data Bank (PDB). This absence probably stems from the limitation of NMR and X-ray crystallographic techniques in their ability to acquire high-resolution structures from proteins as...
These tools and precautions will be useful to address open biological questions: No genetic buffering/redundancy among proton-activated GPCR has been observed in data coming from studies with knockout mice (e.g., deletion of GPR4 does not lead to a compensatory increase in OGR1 expression). Is...
(HS towards LS)5,6,7. Although the alkaline transition has been studied for decades, questions remain regarding the nature of the trigger group that on deprotonation initiates the electronic and conformational change in the native molecule6. Similarly, the hydrogen bonding between Asp and proximal ...
Caught in the act: ATP hydrolysis of an ABC-multidrug transporter followed by real-time magic angle spinning NMR. FEBS Lett. 582, 3557–3562 (2008). 24. van Veen, H. W., Margolles, A., Muller, M., Higgins, C. F. & Konings, W. N. The homodimeric ATP-binding cassette ...
The observations on active drug transport by MsbA-MD raise questions about the functional importance of ATP binding and hydrolysis in full-length MsbA. The direct comparison of the transport activities of MsbA-WT and MsbA-MD in cells show that MsbA-WT catalyses ethidium efflux to lower intracellu...