Cells growing in culture SYTOX Green Nucleic Acid Stain (Cat. No. S7020) Phosphate-free buffer Fluorescence microscope Protocol Labeling fixed cells First, fix and permeabilize cultured cells with a protocol ap
Cells growing in culture ActinRed 555 ReadyProbes Reagent (Cat. No. R37112) Fluorescence microscope Protocol Labeling fixed cells First, fix and permeabilize cultured cells with a protocol appropriate for your sample. Counterstain as desired. DAPI may be added directly to fix...
Centrifuge at 350xg for 5 minutes and discard the supernatant. Wash cells 1X with Cell Staining Buffer and perform cell surface immunofluorescent staining as described above. Fix, permeabilize, and stain intracellular antigens as described above. Flow...
15- Clear in xylene three times, 16- Mount the slide on xylene three times,a) Presence of a psammoma body in absence of atypical cells in cervicovaginal smear (Papanicolaou stain, 200×); b) Serous ovarian cystoadenofibroma with parietal psammoma bodies (Hematoxylin and Eosin, 100×)....
Fix the cells with 4% formaldehyde for 10-15 min at RT Wash cells with PBS for three times. Permeabilize the cells with 1% Triton X-100 for 20min at RT. Wash cells with PBS for three times. Blocking Blocking reduces non-specific binding between antibodies and other proteins that are not...
1. Pellet the exosomes from culture supernatant of HCT116 cells by centrifugation at 100,000 x g for 1.5 h23. Remove the culture supernatant and carefully fix the purified exosome pellet with 1 mL of 2.5% glutaraldehyde in 0.1 M sodium cacodylate solution...
4.4 While cells are incubating in Fc Block, proceed to step 5 – Stain cells with cell surface antibodies.5. Stain cells with cell surface antibodiesNotes: If you plan to multiplex samples using TotalSeq hashtags, there are two commonly used approaches to staining samples with hashtag ...
Do not keep the cells in culture later than passage 20, and do not freeze down again, as another freeze-thaw may alter the cells’ phenotype. We recommend extensive characterization of each new cell line prior to use in functional assays, to ensure a consistent phenotype. We recommend ...
Re-stain cells with proper amount of antibodies and SA-APCCy7. Problem 5 Unstable TMRE histogram (Related to step 4. Sort MMP fractions of CD150+ HSCs). Description: TMRE histogram of CD150+ HSCs keep fluctuating (step 4. g.). Cause: Fewer than 50 parental events (CD150+ HSCs) are...
Fix cells by adding formaldehyde to a final concentration of 1% (add 5.5 mL of 37% formaldehyde to 200 mL of culture medium). 2. Incubate at room temperature (RT) in fume hood for 10 minutes, gently swirl 200 mL culture or invert tube containing 20 mL of adherent cells occasionally to...