First, fix and permeabilize cultured cells with a protocol appropriate for your sample. 1. Wash the cells 1–3 times in PBS as needed. 2. Add sufficient 300 nM DAPI stain solution to cover the cells. 3. Incubate for 1–5 minutes, protected from light. 4. Remove the stain s...
Centrifuge at 350xg for 5 minutes and discard the supernatant. Wash cells 1X with Cell Staining Buffer and perform cell surface immunofluorescent staining as described above. Fix, permeabilize, and stain intracellular antigens as described above. Flow...
Cells growing in culture ActinRed 555 ReadyProbes Reagent (Cat. No. R37112) Fluorescence microscope Protocol Labeling fixed cells First, fix and permeabilize cultured cells with a protocol appropriate for your sample. Counterstain as desired. DAPI may be added directly to fi...
1. Pellet the exosomes from culture supernatant of HCT116 cells by centrifugation at 100,000 x g for 1.5 h23. Remove the culture supernatant and carefully fix the purified exosome pellet with 1 mL of 2.5% glutaraldehyde in 0.1 M sodium cacodylate solution...
15- Clear in xylene three times, 16- Mount the slide on xylene three times,a) Presence of a psammoma body in absence of atypical cells in cervicovaginal smear (Papanicolaou stain, 200×); b) Serous ovarian cystoadenofibroma with parietal psammoma bodies (Hematoxylin and Eosin, 100×)....
1. Pellet the exosomes from culture supernatant of HCT116 cells by centrifugation at 100,000 x g for 1.5 h23. Remove the culture supernatant and carefully fix the purified exosome pellet with 1 mL of 2.5% glutaraldehyde in 0.1 M sodium cacodylate solution (pH 7.0) for 1 h at 4 °C. ...
1. Remove embryos from embryo culture medium and wash 2 times in 100 µl drop of PBS containing 1 mg/ml polyvinylpyrrolidone (PVP) by transferring the embryos from drop to drop. 2. (Optional) Fix embryos in 100 µl drop of paraformaldehyde solution [4% (w/v) in PBS, pH 7.4] fo...
Fix cells by adding formaldehyde to a final concentration of 1% (add 5.5 mL of 37% formaldehyde to 200 mL of culture medium). 2. Incubate at room temperature (RT) in fume hood for 10 minutes, gently swirl 200 mL culture or invert tube containing 20 mL of adherent cells occasionally to...
In this experiment, the biggest trouble for researchers is how to fix the suspension cells to the glass slide. This protocol describes detailed steps for solving this problem. To ensure the cell viability, it is recommended to use cells that have been in culture between 1 week and 1.5 months...
After incubation, Centrifuge cells, pellet and remove supernatant. FIX cells: Add 10ml ice cold 70% Ethanol to a 15ml tube containing the cell pellet, adding dropwise at first while vortexing, mix well. Store at ...