After incubation at 37 1C for 30 min, 2.5 ml (10 mg/ml) propidium iodide (PI) solution was added. The DNA content of PI-stained cells was analyzed using flow cytometry (Becton Dickinson FACSCalibur, BD Biosciences, San Diego, CA, USA). Cell apoptosis assay. MCF7 cells were seeded ...
Global changes in the pentameric receptor are thought to involve a coordinated twist in the five subunits leading to an increase in the channel diameter, thus allowing for ion flow through this enlarged pore [30]. Molecular dynamics simulations of the GPI-anchored lynx1 bound to α4:α4 ...
Right: total RNA from siFra-RAWs and sicon-RAWs was analyzed by real-time PCR in triplicate for the expression of Fra-1 mRNA. The data are expressed as a percentage of the mRNA expression of sicon-RAWs. (C) Flow cytometric analysis of the percent- age of M2d macrophages in the siFra-...
After being washed twice with PBS, cells were incubated with a pre-prepared 500 μL PI/RNase: A staining working solution at room temperature for 30-60 min in the dark. Then the cell cycle was analyzed using flow cytometry and Flowjo software. 2.6 Western blot ESCA cells transfected with ...
We performed an in vitro study involving MTT, flow cytometry, RT-PCR, and western blot analyses to investigate the effects of PCA treatment on cell proliferation, cell cycle distribution, apoptosis, and expression of several cell cycle-related genes in HT-29 cells. The treatment enhanced S-...
Data Error and Incorrect Table Headings in: Patient-Reported Long-term Outcomes After Conventional and High-Dose Combined Proton and Photon Radiation for Early Prostate CancerXFEMtwo-fluid flowenriched methodpartition of unityThe vesper mouse, Nyctomys sumichrasti (Rodentia: Cricetidae), a poorly ...
A HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, then cellular apoptosis was assayed by the flow cytometry. B HepG2 and HuH-7 cells were treated with various doses of protopine for 24 h, then relative caspases activities were measured. C HepG2 ...