Figure 13.2.Schematic overview of mass spectrometry (MS)–based workflow used for proteome profiling of tissue samples and biological fluids. MS-based proteomics can be used for the identification of novel biomarkers or validation and quantification of specific therapeutic targets in the clinic. Proteins...
et al. Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry. Nat. Protoc. 13, 1632–1661 (2018). Article CAS PubMed PubMed Central Google Scholar Christopher, J. A. et al. Subcellular proteomics. Nat....
In this Tools of the Trade article, Zhang and Owyong (Hong lab) discuss the development of a fluorescent probe that binds disordered proteins in situ and allows their enrichment and identification using a mass-spectrometry-based workflow.
Mass-spectrometry (MS)-based single-cell proteomics (SCP) explores cellular heterogeneity by focusing on the functional effectors of the cells鈥攑roteins. However, extracting meaningful biological information from MS data is far from trivial, especially with single cells. Currently, data analysis ...
Move away from bulk sample to single cell proteomics analysis with an Orbitrap mass spectrometry workflow designed in collaboration with the scientific community to identify and quantify proteins at the single cell level.
A workflow for COVID-19 protein biomarkers identification. The workflow for COVID-19 protein biomarker identification using mass spectrometry (MS)-based proteomics. SARS-CoV-2 infects patients with a different precondition, causing different degrees of symptoms. Urine, plasma or serum, and pharyngeal...
In the general workflow, proteins from a biological sample are digested by protease, and separated by liquid chromatography; mass spectrometry is then used to determine their mass-to-ch...关键词:diagnosis drug targets host parasite relationships identification infections mass spectrometry parasites ...
Highly Reproducible Automated Proteomics Sample Preparation Workflow for Quantitative Mass Spectrometry. Fu, Q., et al., Highly Reproducible Automated Proteomics Sample Preparation Workflow for Quantitative Mass Spectrometry. J Proteome Res, 2018. 17(1): p... Q Fu,MP Kowalski,M Mastali,... - Ame...
nf-core/quantmsis a bioinformatics best-practice analysis pipeline for Quantitative Mass Spectrometry (MS). Currently, the workflow supports three major MS-based analytical methods: (i) Data dependant acquisition (DDA) label-free and Isobaric quantitation (e.g. TMT, iTRAQ); (ii) Data independent...
Single-cell proteomics can reveal cellular phenotypic heterogeneity and cell-specific functional networks underlying biological processes. Here, we present a streamlined workflow combining microfluidic chips for all-in-one proteomic sample preparation and data-independent acquisition (DIA) mass spectrometry (MS...