Thermo Scientific Orbitrap mass spectrometers are at the forefront of mass spectrometry technology, capable of accurately identifying and quantifying thousands of proteins with over five orders of linear dynamic range and tight coefficients of variation, even at the single-cell level. These in...
Thermo Scientific Orbitrap mass spectrometers are at the forefront of mass spectrometry technology, capable of accurately identifying and quantifying thousands of proteins with over five orders of linear dynamic range and tight coefficients of variation, even at the single-cell level. These i...
Recent advancements in the profiling of proteomes at the single-cell level necessitate the development of quantitative and versatile platforms, particularly for analyzing rare cells like circulating tumor cells (CTCs). In this chapter, we present an integrated microfluidic chip that utilizes magnetic ...
Their tool was assessed at low sample amounts (10 ng or ∼100 cells). We anticipate that these improvements will be beneficial at the single-cell level. Possible Solutions to Improve Peptide Identification Given the recent emergence of SCP, there are no peptide identification algorithms that ...
Sequencing of nucleic acids with nanopores has emerged as a powerful tool offering rapid readout, high accuracy, low cost and portability. This label-free method for sequencing at the single-molecule level is an achievement on its own. However, nanopores
High-throughput single-cell proteomics quantifies the emergence of macrophage heterogeneity. Preprint at bioRxiv. https://doi.org/10.1101/665307 (2019). Virant-Klun, I., Leicht, S., Hughes, C. & Krijgsveld, J. Identification of maturation-specific proteins by single-cell proteomics of human...
At the same time, existing single-cell proteomics MS methods are reaching a maturity level that should allow their broader adoption. This perspective focuses on key steps needed to achieve broader adoption of single-cell proteomics by tandem MS and to scale up its throughput to tens of thousands...
Proteomics, the study of proteins, provides a comprehensive picture of disease phenotypes and mechanisms at the functional site. Through proteomics at a population scale, scientists can identify causal proteins, uncover novel pathways, and discover potential therapeutic targets. This holistic approach has...
Perioperative Statins at Noncardiac Surgery, Personalized and Precision Medicine & Genomic Research, Phagophore, Pharmaceutical Analytics, Pharmaceutical Drug Discovery, Pharmacodynamics and Pharmacokinetics, Pharmacogenomics, Pharmacotherapy and Cell Activity, Pharmacotherapy of Cardiovascular Disease, Phosphatase, Pho...
The term "protein species" has been defined by Jungblut et al. at the chemical, molecular level [1, 2]. According to this definition, isoforms represent different protein species, because they are also chemically different. In contrast, two proteins with different post-translational modifications ...