WB analysis using 29528-1-AP Untreated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29528-1-AP (Phospho-IRF3 (Ser396) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH ...
Fengjiang Qin, Dan Yang, Chunyan Tang, Yu Yang, Jing Xu, Tao Liu, Ping YiProteintech),IRF3(1:5000,11312‐1‐AP,Proteintech), p‐IRF3(1:1000,29528‐1‐AP,Proteintech),GAPDH(1:10000,60004‐1‐Ig,Proteintech).Membraneswere incubated with the secondary antibody for 1 h at room temperature...