ProteinsBecause of the complexity of cellular material and body fluids, it is seldom possible to analyze a natural product directly. Qualitative and quantitative analyses must often be preceded by some purifi
Full size image Generally, TRIM proteins are E3 ubiquitin ligases based on their RING domain [7]. B-box domains contain one or two different zinc-binding motifs and are divided into 2 types. They promote the catalytic effect of RING or mediate ubiquitination of substrates independently [8, 9...
Selected expression profiling of full-length proteins and their variants in human plasma. Clin. Proteomics - Kiernan, Nedelkov, et al. - 2004 () Citation Context ...a small scale [21–31]. More important, however, was the fact that new PTMs were discovered for several plasma proteins, ...
The brown planthopper (BPH) is a kind of piercing-sucking insect specific to rice, with the damage tops the list of pathogens and insects in recent years. microRNAs (miRNAs) are pivotal regulators of plant–environment interactions, while the mechanism u
However, in the absence of structural data with the full-length Tau protein, the stoichiometry of the complex or the interface and affinity of the partners, are still unclear. In this work, we addressed these points, using a broad range of biophysical techniques. The interaction of the long ...
Table 1 Functions of SARS-CoV-2 Non-structural proteins Full size table SARS-CoV-2 genetic diversity and their clinical implications Sequencing of the whole genome of SARS-CoV-2 revealed that the virus has 96.2% similarity to that of a bat SARS-related coronavirus (SARSr-CoV; RaTG13) colle...
Full size table To quantify the metastability limits of the proteins in terms of the chemical environment, one can assess the energetics of formation reactions for the proteins. At metastable equilibrium, the predominant protein in a population is the one with the highest chemical activity (in this...
coli CusA with the corresponding residues in the CzrA and NczA orthologs Full size table Conclusion In this work, we show a comparison of two HME-RND family efflux systems (czrCBA and nczCBA), where the RND proteins (CzrA and NczA) have the motif DFG-GAD-VEN involved in the export...
Recombinant Fusion ProteinsTumor Suppressor ProteinsCell Cycle ProteinsCrosses, GeneticFlow CytometryProtein DenaturationCyclin-Dependent Kinase Inhibitor p27Nat Med. 1998 Dec;4(12):1449-52. Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S....
Full size image Optimizing the elution protocol to maximize the proportion of monomeric protein At the previous stage, resins were thoroughly dried by centrifugal filtration in order to recover maximal liquid fraction and to quantify all oligomer species that were bound (filter elution protocol). After...