(E3KARP) to the tail of other membrane proteins. The FERM domain also binds to signalling molecules in the Rho pathway, including Rho guanine dinucleotide dissociation domain (Rho-GDI). An F-actin binding site is present in the carboxy-terminal domain of ERM proteins. So far, the F-actin ...
The design of proteins that bind to a specific site on the surface of a target protein using no information other than the three-dimensional structure of the target remains a challenge1–5. Here we describe a general solution to this problem that starts
Two cyclic dinucleotide molecules bind within the cavity of human SLC19A1 as a compact dual-molecule unit, whereas folate and antifolate bind to SLC19A1 as a monomer and occupy a distinct pocket of the cavity. Qixiang Zhang ,Xuyuan Zhang ...
One advantage of protein design is the ability to incorporate multiple functionalities into a signal molecule. One specific resilin polypeptide has been engineered with a cell-adhesive RGD peptide, an MMP-cleavable sequence to enable proteolytic degradation, and a heparin-binding domain for non-covalent...
TDC maintains a resource list that currently contains 22 tasks (and its datasets) related to small molecules and macromolecules, including PPI, DDI and so on. MoleculeNet published a small molecule related benchmark four years ago. In terms of datasets and benchmarks, protein design is far less...
Labeling antibodies and proteins with biotin leverages one of the strongest non-covalent bonds in nature between biotin and streptavidin. Streptavidin has the capacity to bind four biotin molecules, which can dramatically enhance detection of low-abundance proteins. Common applications for biotinylated anti...
Soluble ligands specific for cell surface molecules involved in APC-T cell interactions can signal cells and modulate immune responses. Recently, we reported that LFA3TIP, a fusion protein comprised of the first LFA-3 extracellular domain fused to the hinge, CH2, and CH3 regions of a human IgG...
to the signal. These requirements of cell signaling impose unique, and often conflicting requirements on the proteins that constitute the signaling pathways, such as signal integrity versus crosstalk. These apparent conflicts extend to the individual interactions that propagate the signal, an apparent ...
A protein domain that is commonly found in signal-transduction molecules. It interacts specifically with phosphotyrosine-containing peptides. Lipoxins Leukocyte-derived eicosanoids generated during the inflammatory response that act as downregulatory signals. ...
Photolabeling of intracellular molecules is an invaluable approach to study multiple cellular processes. Here, the authors report on the near-infrared to far-red photoconversion in the miRFP family of fluorescent proteins, which enables photolabeling entirely performed in the near-infrared range. France...