Importantly, activator function through the highly purified f:TFIID complex still requires a general cofactor fraction containing upstream factor stimulatory activity (USA). As previously observed with partially purified activator-competent natural TFIID, f:TFIID generates extended TATA-dependent footprints on...
We found that Mmm1p and Mdm10p exist in a complex with Mdm12p, another integral mitochondrial outer membrane protein required for mitochondrial morphology and inheritance. This interpretation is based on observations that 1) Mdm10p and Mdm12p showed the same localization as Mmm1p; 2) Mdm12p, ...
One of the subunits present in this protein complex is the TATA-binding protein (TBP) as revealed by copurification of TIF-IB activity and TBP over different chromatographic steps including immunoaffinity purification. In addition to TBP, three tightly associated proteins (TAFs-I) with apparent ...
Unfolding of the gene expression program that converts precursor cells to their terminally differentiated counterparts is critically dependent on the nucleosome-remodeling activity of the mammalian SWI/SNF complex. The complex can be powered by either of two alternative ATPases, BRM or BRG1. BRG1 is...
In cells depleted of FtsN, the 1 MDa complex is not assembled. Combined, our findings indicate that a large protein complex containing many different cell division proteins indeed exists. We note that this complex is very fragile and sensitive to the expression of tagged versions of FtsQ. ...
Serum albumin binding studiesMagnetic propertiesThe 1D copper(II) complex {[Cu2(L)2(fum)]·(H2O)·(MeOH)}n(1) has been synthesized using fumarate (fum2−) and a Schiff base (HL), derived from the condensation reaction of 2-amino-1-butanol and salicylaldehyde. Complex1has been ...
We identified 34 high confidence potential binding partners of FHL1. We then showed that FHL1 exists as part of a complex that binds with PDLIM1, GSN and ACTN1. 展开 关键词: High affinity receptor of IgE (Fc epsilon RI Alpha subunit of Fc epsilon RI (Fc epsilon RI alpha Antisense ...
Finally, using Abs against known RNA-binding proteins, we identified the polypyrimidine tract-binding protein (or heterogeneous nuclear ribonucleoprotein I) as a candidate RNA-binding component of complex I. Interaction between CD40 expressed on B cells, macrophages, and other APCs with its ligand ...
2d). Meanwhile, the downregulated proteins are found to be involved in BPs such as mitochondrial respiratory chain complex 1 assembly, muscle contraction, and sarcomere organization, MFs such as protein binding, and metal ion binding, and CCs such as the mitochondria, Z discs, and sarcomeres. ...
This being said, we cannot exclude that nucleic acids already bound by proteins are protected from nuclease cleavage and may contribute to the observed binding events. Characterization of a Tetrahymena SWI/SNF complex The Tetrahymena SWI/SNFTt complex, as defined by the set of proteins that co-...