The average molecular weight for the enzyme in the presence of peptide at the concentration of 8 µM was 97.7 ± 56.6 kDa (R2 = 0.591). Therefore, the average molecular weight for the enzyme in complex with the peptide was decreased when compared to the enzyme in the ...
Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus...
The protein charge under physiological pH was estimated using Protein Calculator v3.4 (http://prot- calc.sourceforge.net/). Protein expression and purification. E. coli Rosetta (DE3) cells harboring the pETSUMO-CnGRASP vector were grown at 310 K in LB medium containing kanamycin (50 ...
The predicted molecular weight is 84.3 kDa and the accession number is A6HDB7. Alias kinase insert domain receptor Chemical Properties Molecular Weight 84.3 kDa (predicted) Storage & Solubility Information Storage Shipping with blue ice. Calculator Molarity Calculator Dilution Calculator Reconstitution ...
(2, 5). Given that its predicted homodimeric molecular weight is 40 kDa, the molecule is presumably glycosylated. In the mature region, mouse IL-17D is 88% aa identical to human IL-17D. There is less than 30% aa identity between mouse IL-17D and other members of the mouse IL-17 ...
Molecular Weight86.2 kDa (predicted). Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. Storage & Solubility Information StorageShipping with blue ice. Calculator Molarity Calculator Dilution Calculator
HiMark Unstained High Molecular Weight (HMW) Protein Standard contains nine proteins in the range of 40–500 kDa designed for analysis of high molecular weight proteins. The protein standard is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add ...
136.35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
For c) and d), black bars correspond to the elution volume of globular protein markers: BSA 66 kDa (1), MBP 45 kDa (2) and Lysozyme 14.3 kDa (3). Black line: SEC profile, red line: measurement of the molecular weight. ...
Theoretical mass of the wild-type protein was calculated using PROTEIN CALCULATOR v3.3 (http://www.scripps.edu/Bcdputnam/protcalc.html), and theoretical mass for the modified protein was adjusted manually. In vivo photocrosslinking of HdeA dimmers. E.coli (DH10B) cells expressing HdeA-F35...