2002. A heterodimerizing leucine zipper coiled coil system for examining the specificity of a position interactions: Amino acids I, V, L, N, A, and K. Biochemistry 41: 14122–14131. Article PubMed CAS Google Scholar Akbergenova Y, Bykhovskaia M. 2007. Synapsin maintains the reserve ...
Near complete 1HN, 15N, 13Cα, 13Cβ and 13C’ backbone resonances of Xph15 have been assigned except for some nuclei within the N-terminal six amino acids, residues of the AB loop, and the C-terminal deca-histidine tag. It was clear during the assignment process that several residues...
In order to insert a peptide fragment into the extracellular domain of P2X2 using tPTS, we used an analogous approach to that described for Nav1.5 and GFP to generate three constructs: (1) an N-terminal construct (N) corresponding to amino acids 1–53 of P2X2 fused to IntNofCfaDnaE;...
Energy and protein intakes are the primary nutritional effectors of protein synthesis. Protein synthesis is downregulated to spare endogenous energy stores during periods of fasting and/or sustained energy deficit.Dietary protein provides the essential amino acids necessary to support the synthesis of new...
Even though CFPS are able to achieve protein glycosylation and incorporation of unnatural amino acids, they still struggle with both the efficiency and homogeneity of proteins with these modifications [133]. As mentioned above, there are advantages to CFPS-based membrane protein synthesis, but there ...
Activated Protein C (390-404), human is a peptide fragment of Vitamin K dependent Serine protease activated protein C (APC), which contains 15 amino acids in its sequence and can inhibit the anticoagulant activity of APC. Purity (HPLC) ≥98.0% ...
Molecular WeightApproximately 34.8 kDa with 6×His-tag and a Cys on C-terminus, a single non-glycosylated polypeptide chain containing 307 amino acids. AA SequenceMNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLN...
Approximately 47.8 kDa with a Cys on N-terminus, a single non-glycosylated polypeptide chain containing 430 amino acids. 氨基酸序列 CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP...
DEKTGLYLLDFEK/NMYKYKKIGNKYVVSINNHTEIVEALKAFCEDKKIKSGTVNGIGQVSKVTLKFFNIETKESKEKTFNKNFEISNLTGFISEINGEVFLHLHITIGDENFSALAGHLLSAVVNGEAILIVEDYKEKVNRKYNEELGLNLLDFNL score- average over residues that were designed negative log probability of sampled amino acids...
global score- average over all residues in all chains negative log probability of sampled/fixed amino acids fixed_chains- chains that were not designed (fixed) designed_chains- chains that were redesigned model_name/CA_model_name- model name that was used to generate results, e.g.v_48_020 ...