1f). Collectively, our CLIP-seq data and EMSA data indicate that LC3B directly binds to RNA with a preference for the AAUAAA motif. LC3B elicits rapid mRNA degradation in response to autophagic activation What happens when LC3B binds to mRNA? To address this question, we assessed the relative...
In our initial characterization of the interaction between EGFR and RanBP6 in A431 cells, we noted that EGF stimulation (5 min, 100 ng/ml) reduced the interaction between RanBP6 and EGFR (Fig.5a). This suggested that RanBP6 might be part on an auto-regulatory mechanism where suppressio...
In cells, S100A8/A9 relies on calcium ions to bind to specific cytoskeletal components such as microtubules, vimentin, keratin, and actin filaments. With the participation of calcium, S100A8/A9 forms a tetramer that directly binds to the intracellular microtubules and promotes the rapid ...
Calmodulin could directly bind to calcium ions as their intracellular recipient, following the Ca2+ influx triggered by alterations on membrane potential. Consequently, the Ca2+-calmodulin complex will stimulate the family of calcium/calmodulin-dependent protein kinase kinases, which consists of calc...
and visualize these structures. The proteins that a given ligand binds are often homologous and present the same binding structure to the ligand. However, there are also many instances wherein a given ligand binds to two or more unrelated proteins, or to the same or homologous protein in diffe...
Ca2+ binds directly the calmodulin domain of CPKs and induces a conformational change resulting in kinase activation [34]. The available information on plant CPKs from various plant species indicates that they are encoded by multigene families and that whereas some of the genes are ubiquitously ...
According to this observation calcium signaling should be implicated in adaptive rearrangement of the cytoskeleton during tardigrade rehydration. The cytoskeleton is a key element in the organisation of eukaryotic cells. It has been described in the literature that the properties of actin are modulated ...
In summary, the protein ICln directly binds the highly conserved (1020)KVGFFKR(1026) amino acids of the intracellular domain of the integrin αIIb via the (61)ISLHA(65) amino acid motif lying in the β4 - β5 loop and β5 sheet of its pleckstrin-homology-like N-terminal portion. The...
Our study further suggests that this apoptosis mechanism can actually be induced by a molecular interaction involved in the allosteric bA domain of b3 integrin. This is important as the notion provides a novel concept to develop integrin avb3 target therapies that would be able to directly remove...
Links between VCP and pathologic inclusions were uncovered by analyzing a superoxide dismutase 1 (SOD1) variant that causes ALS. The E3 ligase Dorfin (double ring finger protein, RNF19A) ubiquitinates SOD1G85R, but not wild type SOD1 [76]. Dorfin binds VCP directly, and VCP-Dorfin complexe...