We isolated a novel full-length human cDNA that encodes a polypeptide with a predicted molecular mass of 21.7 kDa, which binds to the integrin αIIb cytoplasmic domain and is expressed in platelets. The structural properties of CIB indicate that it is a hydrophilic calcium-binding protein, most...
Calmodulin could directly bind to calcium ions as their intracellular recipient, following the Ca2+ influx triggered by alterations on membrane potential. Consequently, the Ca2+-calmodulin complex will stimulate the family of calcium/calmodulin-dependent protein kinase kinases, which consists of calci...
1f). Collectively, our CLIP-seq data and EMSA data indicate that LC3B directly binds to RNA with a preference for the AAUAAA motif. LC3B elicits rapid mRNA degradation in response to autophagic activation What happens when LC3B binds to mRNA? To address this question, we assessed the relative...
In our initial characterization of the interaction between EGFR and RanBP6 in A431 cells, we noted that EGF stimulation (5 min, 100 ng/ml) reduced the interaction between RanBP6 and EGFR (Fig.5a). This suggested that RanBP6 might be part on an auto-regulatory mechanism where suppressio...
In cells, S100A8/A9 relies on calcium ions to bind to specific cytoskeletal components such as microtubules, vimentin, keratin, and actin filaments. With the participation of calcium, S100A8/A9 forms a tetramer that directly binds to the intracellular microtubules and promotes the rapid ...
Polymyxin B is a cyclic polycationic peptide antibiotic (Figure 17.3.3) that binds to lipopolysaccharides and anionic lipids. Polymyxin B is also a selective inhibitor of protein kinase C, with an IC50 of ~35 µM, as well as ...
Ca2+ binds directly the calmodulin domain of CPKs and induces a conformational change resulting in kinase activation [34]. The available information on plant CPKs from various plant species indicates that they are encoded by multigene families and that whereas some of the genes are ubiquitously ...
As a moiety of the RISC, the siRNA binds to the target mRNA in a sequence-specific manner that is mediated by complementary base pairing, leading to cleavage of the target RNA phosphate backbone near the center of the duplex via the action of the RNase-H like activity of Ago-2. An ...
In summary, the protein ICln directly binds the highly conserved (1020)KVGFFKR(1026) amino acids of the intracellular domain of the integrin αIIb via the (61)ISLHA(65) amino acid motif lying in the β4 - β5 loop and β5 sheet of its pleckstrin-homology-like N-terminal portion. The...
discoideum remain to be elucidated, the enzyme DIF dechlorinase that directly binds to and degrades DIF-1 was recently identified to be GST3/DrcA (DIF reductive dechlorinase encoded by the gene drcA, DDB_G0293840) [23]. In addition, experiments using tritium-labeled DIF-1, recombinant ...