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During the initial stages of this study, multiple test samples demonstrated lower intensities as a result of utilizing an un-optimized protocol (data not shown). However, continued editing and updates to the initial procedure on re-utilized H&E index slides resulted in viable stain intensity, ...
To test this model, we used molecular dynamics of membrane-embedded hDHHC20 and its mutants either in the absence or presence of various acyl- CoAs. We found that among a range of acyl chain lengths probed only C16 adopts a conformation suitable for hDHHC20 autoacylation. This specificity ...