" Protein Splicing and Its Applications ", Current Opinion in Biotechnology, 11: 377-383, 2000.Perler, F.B. , and Adam, E. ( 2000 ) Protein splicing and its applications . Curr Opin Biotechnol 11 : 377 – 383 .Perler FB , Adam E ( 2000 ) Protein splicing and its applications . ...
Dissecting the Chemistry of Protein Splicing and Its Applications Angew. Chem. Int. Ed. Engl., 39 (2000), pp. 450-466 View in ScopusGoogle Scholar [2] H. Paulus Protein splicing and related forms of protein autoprocessing Annu. Rev. Biochem., 69 (2000), pp. 447-496 CrossrefView in ...
Many split inteins suffer from poor efficiency in protein trans-splicing, limiting protein engineering applications. Here, authors identify soluble β-sheet-dependent precursor aggregates as the cause, and subsequently use rational mutagenesis to obtain a cysteine-less split intein with full activity. Ch...
Intein-mediated protein splicing has become an essential tool in modern biotechnology. Fundamental progress in the structure and catalytic strategies ofcis-andtrans-splicing inteins has led to the development of modified inteins that promote efficient protein purification, ligation, modification and cyclizati...
(amino acids 1472 to 1502 of NaV1.5 but with N1472 mutated to Cys to enable splicing) flanked N- and C-terminally by IntCofCfaDnaE (IntC-A) and IntNofSspDnaBM86(IntN-B), respectively (Fig.2a). These constructs were transcribed into messenger RNA (mRNA) and injected intoXenopus ...
Moreover,S. cerevisiaecan express heterologous proteins as up to 49.3% (w/w) of its own protein [5].S. cerevisiaehas been commonly documented to produce heterologous proteins, and its current status is summarized in Table1. It has been widely used for producing medicinal proteins, food protei...
Beta Galactosidase encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydr...
Targeted protein degradation (TPD) is an emerging therapeutic strategy with the potential to modulate disease-associated proteins that have previously been considered undruggable, by employing the host destruction machinery. The exploration and discovery
Multiple protein isoforms, containing distinct amino acid (AA) sequences, can arise from the same gene through mechanisms such as alternative promoter usage or splicing [2] and can exhibit different stabilities, molecular binding capabilities, and functional effects [3, 4]. Many protein isoforms ...
proteintrans-splicing; split intein.IntroductionUnderstanding the structure-function relationship of proteinsis crucial for both basic research and applications in bio-technology and biomedicine. Recombinant DNA technologyhas advanced the expression of proteins in heterologous sys-tems likeEscherichia coli(E. ...