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After removal of these, 50 μl of lysis buffer was added to each well, and plates were incubated for 30 min on an orbital shaker at room temperature. 16 μl of cell lysates were transferred to a white 384-well proxi-plate and incubated in the dark for 2 h at room temperature with ...
Overexpression. Transformed cells containing the plasmid pET28a with sty3178 gene are grown in 5 ml Luria-Bertani (LB) medium overnight at 37 °C with constant shaking at 250 rpm in a shaker (Innova 42 New Brunswick Scientific). 1% of starter culture is used to inoculate 1 ...
After labeling, cells were rinsed three times with PBS and incubated on a shaker at RT for 20–30 min. Afterward, the FLAG tag was labeled by immunocytochemistry. Pulse–chase click chemistry labeling of two NFL populations in neurons Neurons were labeled with the first tetrazine dye (BODIPY...
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aTwo loops from yeast slants were used to inoculate 100 ml of the pre-culture medium in 250 ml Erlenmeyer flasks and cultivated on a rotary shaker (180 rpm) at 37 °C for 20 h. 正在翻译,请等待...[translate] afor lighter 为 更轻[translate] ...
After that, 50 μL of 40 % v/v protein A-Sepharose 4B FF in RIA buffer were added and incubated for 2 h at RT on an end-over-end shaker. Subsequently, samples were allowed to settle and the supernatants were discarded in order to isolate immunocomplexes. Pellets were washed three ...
TiO2 beads (0.6 mg per 100 µg peptide solution) were added, mixed well, and placed on a shaker for 15 min at room temperature. The solution was then table centrifuged to pellet the beads, which were subsequently mixed with 100 µL loading buffer, transferred to LoBind tube...
(r-protein), i.e., L23, in the vicinity of the ribosomal exit tunnel. This result is surprising because the ribosomal surface near the tunnel exit bears several r-proteins. A wider interaction network, including one additional ribosomal protein and the trigger factor (TF) chaperone, gets ...
200 μL of the LB culture was inoculated into 20 mL SM10+ media with appropriate antibiotics in 250 ml shaker flasks. The culture was incubated at 37° C. with a shaking speed of 150 rpm for 16 hours, at which time OD600 nm is usually between 6 and 10. The culture was harvested ...