Using PGAP-controlled gene expression, NRRL Y-11430 has somewhat more host cell proteins in its culture supernatant and grows to a lower cell culture density (in shake flasks) compared with the type strain. We hypothesize that both observations are related and due to slightly increased cell lysis...
In order to shift the dynamics of GNCA towards TEM-1 β-lactamase, we aim to use a rational design principle of the hinge-shift mechanism where we attempt to reproduce the shift in hinge locations made through evolution. We, therefore, substitute the positions of GNCA residues with the corr...
The lining of the cellulose-binding path is identical in the three enzymes except at two locations, loop A1 at the entrance to the tunnel and loop A3 near the catalytic center (Fig. 6). In loop A1, Glu101 in TreCel7A binds the 6-hydroxyl of the glucose unit in subsite − 6. This...
In order to shift the dynamics of GNCA towards TEM-1 β-lactamase, we aim to use a rational design principle of the hinge-shift mechanism where we attempt to reproduce the shift in hinge locations made through evolution. We, therefore, substitute the positions of GNCA residues with the corr...
The lining of the cellulose-binding path is identical in the three enzymes except at two locations, loop A1 at the entrance to the tunnel and loop A3 near the catalytic center (Fig.6). In loop A1, Glu101 inTreCel7A binds the 6-hydroxyl of the glucose unit in subsite − 6. This ...
2d are predicted to be aggregation prone14, which provides a basis for the effect of ADH-6 on sensitive locations other than p53251–258 in p53 DBD. Chemical shift changes, however, may also be the consequence of local rearrangements in response to an allosteric interaction. Therefore, only ...
To explore their locations and dynamic aspects, specific binding of noble gases, particularly xenon, into protein cavities has been studied by X-ray crystallography8–10. In addition, small organic compounds and paramagnetic agents as well as noble gases have been used as probes in various nuclear...
The SHAKE algorithm was applied to all bonds and an integration step of 2.0 fs was used throughout. The molecular graphics program PyMOL version 1.8.1.060 was employed for visualization and molecular editing. Generation of polyclonal anti-CbpL antisera. Antibodies against rCbpL were raised ...
2d are predicted to be aggregation prone14, which provides a basis for the effect of ADH-6 on sensitive locations other than p53251–258 in p53 DBD. Chemical shift changes, however, may also be the consequence of local rearrangements in response to an allosteric interaction. Therefore, only ...
coli when grown in shake flasks and lysed by sonication [10]. V. natriegens extract has even been shown to maintain 100% of activity after one week of storage at room temperature post-lyophilization in the presence of trehalose [10]. Although this platform appears to be promising in terms...