Get tips and helpful information when starting with your protein purification experiment from choosing the right purification strategy to finding the best tools for your workflow from our broad selection of resins and ...
A cofactor required for activity was removed during purification Add cofactor Table 6. Troubleshooting affinity chromatography. Summarized from GE. Ion exchange chromatography (IEX) Ion exchange chromatography (IEX) separates proteins based on their net surface charge, through electrostatic interactions that...
(bait protein) may be required to ensure that the polyclonal antibody does not contain clones or contaminants that bind prey protein(s) directly. Pre-adsorption to extracts devoid of target or pre-purification of polyc...
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two ...
Proteins are used as reagents in a broad range of scientific fields. The reliability and reproducibility of experimental data will largely depend on the quality of the (recombinant) proteins and, consequently, these should undergo thorough structural and
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3.Troubleshooting 4. General Information 1.Product Description 1.1Intended Use GenScript Protein A/G MagBeads are ideal for smallヽale antibody purification and immunoprecipitation (IP) of proteins, protein complexes or other antigens. 1.2Principle The sample containing antibody is added to the ...
i53 variant protein production and purification The sequences of different i53 variants were cloned into bacterial expression plasmids, resulting in a N-terminal His-tagged fusion protein with a protease cleavage site in between the 6x-His-tag and i53 variant sequence. The resulting plasmids were...
We detail the S-, 2×FLAG-, and Streptavidin-Binding Peptide (SBP)- tandem tags (SFB-tag) system for protein purification. This protocol can be used to identify protein interactors and establish a high-confidence protein–protein interaction network based on computational models. This is ...
Protein purification. The RNA-binding domain of yeast Puf5p (residues 201–600) was subcloned into the pSMX vector with an N-terminal His6-SUMO tag40. Escherichia coli cells BL21 Star (DE3) carrying the Puf5p plasmid were grown in Terrific Broth media to OD600 ¼ B0.8 and then ...