Next, we focus on analysis of B-factors, which characterize the spread of electron density in the crystal. B-factors include contributions from protein structural mobility and lattice disorder20,51. In simulations, B-factors can be computed from the root-mean-squared fluctuation (RMSF) of atomic...
cross-link mass-shift 195.126); fixed modification C 57.021; peptide length, minimum 6 amino acids and maximum 60 amino acids per chain; peptide mass, minimum 600 and maximum 6000 Da per chain; enzyme, trypsin or trypsin and Asp-N, with up to three missed cleavage sites per cross-link...
In Eq.2, RS is the energy of the structure obtained from the Rosetta REF2015 score function. The IMScore_Termterm is a penalty function (as defined and shown in Eq.3and Supplementary Fig.7, respectively) based on the absolute difference (ΔCCS) between CCSPARCSand CCSIM. This function i...
These structures are some of the largest determined by X-ray crystallography, and dissection of the electron density maps has only been possible with the aid of the large body of biochemical and other data accumulated on the ribosome and its subunits. It is remarkable that the secondary ...
7, 8 Indeed, fatty acids and their derivatives, particularly those with long hydrocarbon chains, are ideally suitable as fuel for today’s diesel engines, because they have the highest energy density per unit volume.9, 10 Thus, fatty acid synthase (FAS), the master enzyme of fatty acid ...
Feed input may be reduced in shrimp aquaculture by increasing the protein density of the diet modifying the feeding rate so as to deliver the desired amoun... Jesus,A.,Venero,... - 《Aquaculture》 被引量: 41发表: 2007年 Variable feed allowance with constant protein input for channel catfis...
HCD MS/MS spectra were col- lected for the 15 most intense ions per MS scan at 30,000 resolution with a normalised collision energy of 28% and an isolation window of 1.4 m/z. Dynamic exclusion parameters were set as follows: exclude iso- tope on, duration 30 s and peptide match ...
CHO-K1stably expressing HTs were platted in a 6 well plate at low density (5 × 104cells) and cell confluence imaged with the 10× objective every 4 h using the phase-contrast channel of the Sartorius Incucyte live-cell imaging system (Göttingen, Germany). Exponential growth was ...
In addition, the manual crafted feature vectors do not scale well with graph density and hence cannot be evaluated for larger angstrom thresholds. Evaluation metrics shown in Fig. 2b include cluster-adjusted mutual information and triplet-scoring AUPR. Each benchmark was applied to the top five ...
coli to have a diffusion coefficient that is significantly (100-fold) lower due to a rise in protein density and subsequent increase in viscosity (Brass et al., 1986). 2.1 What are ESRs? Stresses that inflict specific damage to the Gram-negative cell envelope, disrupting periplasmic homeostasis...