Monoclonal antibodies specific for this protein were made by fusing spleen cells from immunised BALB/c mice with the mouse myeloma cell lines SP2/0 or MPC/11. About 10% of cultures which contained hybridomas secreted antibodies to band 4 protein, and of these, 2 high producers were selected ...
Determination of plasma proteins seldom leads to a specific diagnosis (e.g., monoclonal gammopathies) but will help the clinician to evaluate the nature, severity, and progress of a disease. B Plasma versus Serum In pigeons, the concentration of total protein (TP) in plasma is about 1.5 g...
Agaroseor capillaryprotein electrophoresishas adetection limitfor monoclonal proteins of 0.5–1g/L while immunofixation detects bands down to the 50–100mg/L range. Immunofixation therefore defines theabsenceof a monoclonal band and classifies therapeutic response categories[1]. ...
These proteins were precipitated by DRP1-specific monoclonal antibodies and Protein G Sepharose, and characterized by MALDI–TOF. The peptide mass fingerprint of the 80 kDa protein matched that of DRP1: MS-Fit search (http://prospector.ucsf.edu/): O00429, dynamin-related protein 1. (b) The...
Lysosomal-Associated Membrane Protein 2 (LAMP2) 抗体应用备注Flow Cytometry (Neat-1/10): Use 10 μL of the suggested working dilution to label 10^6 cells in 100?μL. Results may be enhanced using permeabilisation, The Fc region of monoclonal antibodies may bind non-specifically to cells ex...
Fully human monoclonal antibodies antagonizing the glucagon receptor improve glucose homeostasis in mice and monkeys. J Pharmacol Exp Ther. 2009;329(1):102–11. https://doi.org/10.1124/jpet.108.147009. Article PubMed CAS Google Scholar Konitzer JD, Pramanick S, Pan Q, Augustin R, Bandholtz ...
Western blot screening of stable lines using a monoclonal anti-FLAG antibody revealed a single 40 kDa band signal, while no signal was detected in control cells (Figure 2a). This is comparable to the theoretical 42 kDa size of the D-BTBD1- FLAG protein. Anti-FLAG immunofluorescence ...
The sections were incubated with a monoclonal rabbit anti-Ki67 antibody (Abcam, Cambridge, UK) or anti-OLFM4 antibody (Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight. The sections were incubated with biotinylated goat anti-rabbit IgG secondary antibody (Vector Laboratories, ...
A relationship of MAG to other adhesion proteins also is demonstrated by the presence in most species of a sulfate-containing epitope in its oligosaccharide moieties that reacts with the HNK-1 monoclonal antibody. The carbohydrate HNK-1 epitope is expressed on many neural adhesion proteins, ...
(Sigma-Aldrich) diluted 1:5000 or rat monoclonal anti-PknB antibodies (1652A rat antibody raised against the catalytic domain; gift from the Statens Serum Institut); HRP-conjugated goat antibodies against mouse (diluted 1:10000; Bio-Rad) or rat (diluted 1:20000; Bio-Rad) were used as ...