We reported an integrated database of Compendium of Protein Lysine Modifications (CPLM; http://cplm.biocuckoo.org) for protein lysine modifications (PLMs), which occur at active ϵ-amino groups of specific lysine residues in proteins and are critical for orchestrating various biological pr...
CPLM: A database of protein lysine modificationsdoi:10.1093/nar/gkt1093Zexian LiuYongbo WangTianshun GaoYu Xue
[35]. The charge–charge interaction is applicable between the two oppositely charged species, ligand functional groups, or protein side chains, carboxyl group, phosphate groups, glutamate side chain are examples of negatively charged species while amine or imine groups, lysine, arginine, histidine ...
Intact MassApplication NoteAntibody Subunit Analysis Workflow on a Quadrupole-Orbitrap Mass Spectrometer: From Optimized Sample Preparation to Data AnalysisWorkflow Intact Protein AnalysisApplication BriefHigh Salt Gradient Analysis of Post-Translational M...
. dbPSP: a curated database for protein phosphorylation sites in prokaryotes.2015, bav031 (2015). Nucleic Acids Res47, D506–D515 (2019). ArticleCAS Huang, K. Y.et al. dbPTM in 2019: exploring disease association and cross-talk of post-translational modifications., D298–D308 (2019)....
By searching for the database of protein modifications for mass spectrometry (UNIMOD)24, the substitution of Lys238 with Glu residue and the conversion of the ε-amino group of Lys238 into a hydroxyl group (conversion of lysine to 6-hydroxy-l-norleucine) were proposed as candidates accounting...
action of proteases was responsible for amino acid polymerization. Typically, proteases cleavepeptide bondsthat link specific paired amino acids. For instance, cleavage by trypsin occurs at the carboxyl side of lysine or arginine, except when either is adjacent toproline. It was claimed that these ...
The lamins are the major architectural proteins of the animal cell nucleus. Lamins line the inside of the nuclear membrane, where they provide a platform for the binding of proteins and chromatin and confer mechanical stability. They have been implicated
Source data are provided as a Source Data file. Full size image The downstream enzyme of PdxB is SerC, a PLP-dependent aminotransferase that plays roles in the biosynthesis of PN, serine, and lysine by using different substrates75,76. The redundancy and promiscuity of SerC have been widely ...
Together, these findings support the idea that SIRT5 is not a physiological deacetylase, and instead removes a class of carboxyacyl-lysine modifications. Our proteomic analyses of Kglu in SIRT5KO mouse liver led to the identification of 683 Kglu sites on 191 proteins. Interestingly, over three...