We characterized the activation of p300 acetyltransferase by the protein cofactor, Mastermind. Taken together, this work encompasses several developments in biochemical tools for the study of protein lysine acetylation and furthers our understanding of the p300/CBP acetyltransferase....
Structure and chemistry of the p300/CBP and Rtt109 histone acetyltransferases: implications for histone acetyltransferase evolution and function. Curr. Opin. Struct. Biol. 18, 741–747 (2008). Article PubMed PubMed Central CAS Google Scholar Yamauchi, T. et al. Increased insulin sensitivity ...
Subcellular organelle proteins from a rat liver peroxisomes-enriched fraction separated by a linear density gradient were analyzed by Western blot using acetylated-lysine-specific antibody. A single 80kDa band was identified in an area where the peroxisomal enzymatic marker catalase localized. LC-MS/MS...
Acetylation of poly (ADP-ribose) polymerase-1 by p300/CREB-binding protein regulates coactivation of NF-κB-dependent transcription. J Biol Chem. 2005;280:40450–64. Article CAS PubMed Google Scholar Brenner JC, Feng FY, Han S, Patel S, Goyal SV, Bou-Maroun LM, et al. PARP-1 ...
75 The evolutionarily conserved Gαi/LGN/NuMA network is particularly important as a regulator of spindle positioning,76 and during the early stages of mitosis, the dynamic interactions between kinetochores and microtubules are driven by p300/CBP-associated factor-mediated K220-EB1 acetylation.77 In...
Lysine acetylation is a way of controlling enzyme activity. Its role may be to control the activity of the existing enzyme pool rapidly without the need for protein synthesis or degradation. Acknowledgments The present work was fully funded by the Nestlé Institute of Health Sciences....
Table 1 Characteristics and functions of lysine acylations Full size table Crotonylation Characteristics and functions of Kcr Scientists discovered that Kcr, a novel histone mark, functions distinctively from Kac [20]. Notably, Kcr can be modulated enzymatically by P300/CBP with sodium crotonate serving...
Lysineacetylationtargetsproteincomplexesandco-regulatesmajorcellular functions ChunaramChoudhary,ChanchalKumar,FlorianGnad,MichaelL.Nielsen,Michael Rehman,TobiasWalther,JesperV.Olsen,MatthiasMann* ThisSOMcontains: Experimentalprocedures Sevensupplementaryfigure(FigureS1-S9)andfigurelegends ...
The cyclic AMP-responsive element-binding protein (CREB) binding protein (CBP, also known as KAT3A) is a cellular paralog of p300. p300 and CBP share extensive sequence identity and functional similarity and are often referred to as CBP/p300. CBP/p300-catalyzed acetylation of histones and ...
Furthermore, mutation of these lysine residues to arginine markedly decreased the synergistic activation of he HIV promoter by Tat and p300 or by Tat and cyclin T1. These results demonstrate that acetylation of Tat by p300/CBP is important for its transcriptional activation of the HIV promoter. ...