DNA breaks can cause mutations, contributing to the formation of tumors. One key protein required for mediating DNA repair is the tumor suppressor 53BP1. Recent studies now demonstrate the crucial role of K48-linked ubiquitination and protein degradation for 53BP1 recruitment at sites of DNA ...
inflammasomeK48-linked ubiquitinationproteasomal degradationUSP3Inflammasomes are essential components of the innate immune system and its defense against infections,whereas the dysregulation of inflammasome activation has a detrimental effect on human health.The activation of inflammasomes is subjected to tight...
DengueNS1 undergoes modification by K48-linkedpolyubiquitinchains. • K189, a residue previously shown to be important for efficientDENVreplication, is anubiquitinationsite on DENV-NS1. • Ubiquitin deconjugation of NS1 reduces NS1 interaction with theviral proteinNS4B. ...
b–d, Misfolded luciferasets (Lucts) confined to the nucleus can be cleared by San1-mediated K48-linked ubiquitination. b, The increase in the percentage of cells containing puncta of NLS–GFP–Lucts and NES–GFP–Lucts across the E3 single- and double-deletion strains is similar to the...
Figure 7. DPV UL24 initiates the K48-/K63-linked ubiquitination of IRF7. (A) The UL24 protein induces IRF7 ubiquitination. Left image: UL24-3Flag, IRF7-Myc and HA-Ub were co-transfected into DEFs, and the samples were collected 36 h later for Co-IP with a mouse anti-Myc antibody...
2A). We further examined the effects of PPARα activation on K63-linked and K48-linked ubiquitination, two types of YAP ubiquitination modifications. Upon WY-14643 treatment, the K48-linked ubiquitination of YAP was reduced (Fig. 2B), whereas the K63-linked ubiquitination of YAP was ...
In sharp contrast to prolyl isomerase Pin that has been shown to promote degradation of FBW7 through increasing self-ubiquitination [29], MDM2 accelerates FBW7 protein turnover by directly augmenting K48 polyubiquitination at Lys412 residue. To this end, MDM2 is recognized as an E3 ubiquitin ...
Post-translational modifications, including glycosylation, ubiquitination, and phosphorylation, are key steps in regulating PD-1 function. The deglycosylation of the PD-1 protein, specifically the removal of N-linked glycosylation, is a critical step for its effective ubiquitination and subsequent degradati...
Our results revealed that GPX4 linear ubiquitination, which was upregulated by OTULIN insufficiency, did not promote GPX4 protein stabilization; however, the concomitant increase in K48-linked ubiquitin on GPX4 tended to promote the proteasomal degradation of GPX4. Owing to the specificity of the ...
7b). And RNF13 knockdown by small interfering RNA (siRNF13) hampered the K48-linked ubiquitination of STING, which can be restored by TRIM29 overexpression (Fig. 9d). Moreover, adenovirus-mediated TRIM29 overexpression blocked the upregulation of STING (Fig. 9e), the intensified lipid ...