A systematic classification of π-turns of both hydrogen-bonded and non-hydrogen-bonded types is necessary in the light of what has been done for shorter α-turns for a proper appraisal of turns in protein structures. By conventional definition π-turns are six-residue long, in which the CO...
On the individual protein level, isolation methods, such as co-immunoprecipitation17, pull-down18, and crosslinking19 are used to establish the structures of individual proteins, so that surficial ‘hotspots’ can be located and analyzed. In short, hierarchy knowledge of structure information is ...
Expression of protein kinases either for facilitated gene deletion mutant or addback generation was achieved by using the pNUS system67 modified for expression with a C-terminal 6xMYC fusion. Gibson Assembly (NEBuilder® HiFi DNA Assembly) was used. The pNUS backbone plasmid was digested using ...
Using the same approach, more than 3,000 different acetylation sites in approximately 2,000 distinct proteins were identified in liver mitochondria and tentatively related with metabolic reprogramming[167]. Combined fractional diagonal chromatography (COFRADIC) was specifically developed for the isolation ...
Protein Quality Control, the Ubiquitin Proteasome System, and Autophagy Christopher C.Glembotski, inJournal of Molecular and Cellular Cardiology, 2014 2The SR/ER as a major site of protein synthesis It has long been believed that about 2/3 of proteins are translated on cytosolic ribosomes, whil...
Protein isolation and Western blot analysis The extraction of total protein and Western blot analysis was conducted with standard protocols as previously described [31, 48]. Uterine tissue proteins were isolated by homogenization in radioimmunoprecipitation assay buffer (Sigma-Aldrich) supplemented with cOm...
Acinetobacter baumannii is a ubiquitous bacteria that is increasingly becoming a formidable nosocomial pathogen. Due to its clinical relevance, studies on
Mason MG, Botella JR: Isolation of a novel G-protein γ-subunit from arabidopsis thaliana and its interaction with Gβ. Biochim Biophys Acta. 2001, 1520: 147-153. 10.1016/S0167-4781(01)00262-7. Article PubMed CAS Google Scholar Thung L, Trusov Y, Chakravorty D, Botella JR: Gγ1+G...
The total volume of each sample was injected onto an Acquity UPLC BEH C18 column (2.1-mm inner diameter × 150 mm; 1.7-μm particle size) on an Acquity UPLC System with a diode array detector (Waters), and the peptides were eluted from the column using a linear gradient of 4...
Ninety-six-well plates were coated with 0.05 mg ml−1poly-d-lysine (BD 354210) one day prior to isolation. A dissection solution was made using HBSS (ThermoFisher 14025092) supplemented with 10 mM HEPES (ThermoFisher 15630080), 33 mMd-glucose (Sigma-Aldrich G8270) and 43 mM...