(a) 293T cells were transiently cotransfected in 12-well plates with GFP reporter pHH21-NP- UTRhi-eGFP together with variable combina- tions of influenza virus expression constructs (pHW181-PB2, pHW182-PB1, pHW183- PA, pHW184-HA, pHW185-NP, pHW186- NA, pHW187-M, pHW188-NS; ...
Targeted protein degradation refers to the use of small molecules to induce the selective degradation of proteins. In its most common form, this degradation is achieved through ligand-mediated neo-interactions between ubiquitin E3 ligases — the principa
Reversible protein phosphorylation, catalyzed by protein kinases and phosphatases, is involved in almost all key events of cell metabolism and signaling in eukaryotes. A wide array of protein kinases and phosphatases have been identified as crucial players in ABA signaling, among which Ca2+-dependent ...
Protein–protein interactions (PPIs) have pivotal roles in life processes. The studies showed that aberrant PPIs are associated with various diseases, including cancer, infectious diseases, and neurodegenerative diseases. Therefore, targeting PPIs is a direction in treating diseases and an essential strate...
Protein–protein interactions(PPIs)have pivotal roles in life processes.The studies showed that aberrant PPIs are associated with various diseases,including cancer,infectious diseases,and neurodegenerative diseases.Therefore,targeting PPIs is a direction in treating diseases and an essential strategy for the...
Variation in the gene encoding dysbindin-1 (i.e., dystrobrevin-binding protein 1: DTNBP1) has frequently been associated with schizophrenia. Several studies have also found that dysbindin-1 gene and protein expression are altered in two affected brain ar
FE Boas,PB Harbury - 《Journal of Molecular Biology》 被引量: 77发表: 2008年 A Conserved Protonation-Induced Switch can Trigger "Ionic-Lock" Formation in Adrenergic Receptors The mechanism of signal transduction in G-protein-coupled receptors (GPCRs) is a crucial step in cell signaling. However...
The quality of predictions in the case of unbound docking varies, depending on the level of structural deviation from bound geometries. We analyze the role of specific protein-DNA interactions on force field performance, both with respect to complex structure prediction, and the reproduction of ...
REGN10987: a patient-derived antibody that binds early variants of SARS-CoV-2 Spike32and that had an FDA EUA for use against these variants C143: an unmatured, patient-derived antibody that binds the SARS-CoV-2 Wuhan-Hu-1 Spike but was isolated before extensive in vivo somatic hypermutation...
In the other arm, the two orthogonal polarizations contain interferograms with the corresponding phase shifts of π and 3π/2. The individual interferograms are then split by two polarizing beamsplitters (PBSs) and imaged onto four individual cameras, which are read synchronously. Fig. 1: Total...