In addition, this cost-effective probe allowed for the rapid and simple determination of Cu2+ions in not only natural water samples but also in a complex biological sample (in this case, blood sample). 展开 关键词: pyridine-functionalized mesoporous silica py-SBA-15 separation and ...
Discover protein biomarkers that matter using workflows for both unbiased discovery-based (untargeted) and targeted determination of changes in protein abundance. Sample Separation Streamline your workflows with a variety of sophisticated separation solutions to improve your application and workflow performance...
There is an unmet need to reliably and non-invasively monitor disease progression in preclinical Huntington’s disease (HD) models. As a marker of axonal damage, neurofilament light chain (NfL) has been suggested a marker for neurodegeneration. NfL concentrations in blood and CSF were recently sho...
6). It is possible that hydrazine may quench the reaction in <5 s, but with manual pipetting we were unable to test shorter time points. Compared to a 10-min cross-linking reaction, a 10-s reduced the number of DOPA2 cross-link spectra by about two-thirds on the BSA sample (Fig...
Specific sample sizes are denoted in the figure legends. Several housekeeping genes, including Gapdh, Actb (β-actin), and L19, were tested before analysis. However, only Gapdh and Actb were stably expressed between the groups and thus used as the reference gene for our analysis. Fold changes...
These vesicles were observed from parasites incubated in secretion medium as well as from parasites directly isolated from the blood of infected rat (Figure 7). To further verify the putative nature of the vesicles present in the sample, a 140,000 g centrifuged pellet fraction from the secretome...
The sample size was based on the assumption that 76 participants across all 4 groups would provide 80% power to detect a standardized treatment difference of 0.65 between those receiving 0.8 g/kg/d of protein and those receiving 1.3 g/kg/d of protein. This difference was deemed clinically mea...
In this regard, correlative methods such as CLEM fill both technical and conceptual gaps that exist within a single imaging technique (Figure 2). CLEM is a broad category of methods where any type of light microscopy and electron microscopy are combined on the same sample and is thus difficult...
We found that BMP7 was overexpressed in the progressed sample (PD) compared with primary tumor (pretreatment) (Fig. 1h–j). Collectively, our findings show that tumors resistant to anti-PD1 had upregulated BMP7 expression and secretion and suggest that its overexpression may promote resistance ...
We thank H. Zhang for the critical suggestions, Y. Zhou and S. Li for their help in the sequence analysis, Y. Huang and X. Cui for the blood sample collection. We thank the Center for Instrumental Analysis and Metrology of Wuhan Institute of Virology for providing technical assistance. Thi...