DNA–protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these interactions on a genome scale in bacteria are laborious and capture only a snaps
Charge distribution in membrane proteins is used to control protein topology and stability in vivo37, for example, the ‘positive-inside’ rule, which modulates the orientation of proteins in the plasma membrane/bacterial inner membrane38,39. By contrast, the ‘positive-outside’ rule for OMPs, ...
This silver stain detects 100 pg quantities of proteins. In order to stain quickly, sensitively, and sharply a protein matrix in a gel, the repeated shrinkage and swelling gel was developed with a hyper- and hypotonic solution to remove the sodium dodecyl sulfate (SDS) from SDS-protein ...
Proteins are highly complex substances present in all living organisms that are of great nutritional value and that are directly involved in the chemical processes essential for life.
Sign in to download hi-res image Fig. 5.Quantification of cell-free protein synthesis examples. (A) Data are representative of quantification by radioactivity. SDS-PAGE autoradiogram shows each protein produced by cell-free protein synthesis. Each column is a separate cell-free reaction producing...
In general, there are four major types of cell surface display proteins in Gram-positive bacteria, which are as follows: proteins anchored to the cytoplasmic membrane by hydrophobic transmembrane domains; lipoproteins that are covalently attached to membrane lipids after cleavage by signal peptides II...
[34] designed 10 RBS sequences with different expression intensities for expressing T7RNAP using an RBS calculator, which was successfully implemented in five Gram-negative and one Gram-positive bacteria. To further extend the regulatory range, Li et al. [35] constructed a more extensive RBS ...
Phenolic compounds are ubiquitous plant secondary metabolites that possess various biological activities and are known to interact with proteins, altering their structure and properties. Therefore, interactions between these compounds and proteins has ga
The medicaments contain a therapeutically effective quantity of one or more S100 protein(s) or one or more mutant or fragments of the same, or contain one or more nucleic acid sequence(s) which code(s) for these amino acid sequences and which are optionally integrated in one or more gene...
Five concentrations of each Vip3 protein (partially purified by using fractions enriched for the Vip protein) were used in preparing artificial MPB diets: 0, 3, 10, 30 and 100 µg of Vip3 protein per gram of diet. Diets with Cry2Aa protein and without Bt protein represented positive ...