abs04717 Human ACE2 Protein (His Tag) 1mg 25000.00 立即咨询 产品更多信息请进入爱必信网站咨询 公司简介 爱必信(上海)生物科技有限公司是一家从事生物学试剂及耗材的高新技术企业。公司秉承“为生命科学创造便利!为客户创造价值!为员工创造幸福人生!”的发展理念,拥有专业的产品研发质检团队、市场营销团队及先进...
TheSaccharomyces cerevisiaestrains are derivatives of the S288C strain BY4742 (Genotype: MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0). Yeast strains used in this study are listed with genotype in Supplementary Table2. The knock-in yeast strains with chromosomally integrated mCherry or SULI were ...
(b) Densitometric analysis of the anti- GFP and anti-His Western blots. Relative TGR5-TGR5 interaction was determined as relative GFP to His levels. Results are expressed as mean + SEM (n = 4), *Significantly different from wt-His/wt-YFP interaction, p < ...
(50 mM NaCl, 10 mM Tris, pH 7.4, 1 mM MgCl2, 0.5 mM EDTA), the DNA-binding protein (one may use between 1 and 10 nM as a starting point depending on the affinity of the protein to DNA), 0.25% milk for protein stabilization, and a 1:500 dilution of a Cy5-labeled anti-His ...
After purification and cleavage of the His-tagged protein, Cres_0567 was examined for channel forming ability. We could indeed demonstrate here that it has the same function as PorAcoram because it is also a channel-forming protein. Our experiments showed that the native PorAcres (Cres_0567) ...
N- or C-terminal truncated agsA genes were amplified from pUHE212-1 agsA using the primers shown in Table 3.ΔN7 F and his-tag AgsA R were used for the N-terminal 7 AA truncation of the agsA gene, ΔN11 F and his-tag AgsA R for the N-terminal 11 AA truncation, ΔN17 F and...
The theoretical molecular mass of LTBMUC1 is 17.7 kDa, but on the His-tag blots the recombinant protein migrated as a single ~20 kDa band (Fig. 6B, left side, upper panel). An ~20 kDa band was also detected upon expression of LTBMUC1 in control, nontransgenic N. benthamiana L. plan...
Acknowledgments The authors would like to thank Dr Peter Lock from the La Trobe Institute for Molecular Science BioImaging Platform for his help with the TEM imaging. Disclosure statement The authors have no conflict of interest. Funding This work was supported by a grant awarded to MAA; ...
C9orf72-FL, RO60-FL, SMCR8-V5, and WDR41-FL constructs were generated by PCR-amplification of Ultimate ORF cDNA clones, using a primer with AAG linker and C-terminal FLAG- or V5-tag, and cloning of the products in pcDNA6/myc-His B (pcDNA6, Invitrogen). Cell line and tissue ...
In order to express proteins with N-terminal His10-tag, pET-24a(+) was modified between NdeI/XhoI sites to generate pET-IG that presents from 5′ to 3′, after the start codon and in frame, a His10-tag, the Tobacco Etch Virus (TEV) cleavage site (-ENLYFQG-) and a multiple ...