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Total protein was incubated with 50 μl of Protein G-agarose suspension (Millipore, USA). After supplementation for 2 h at 4 °C, the beads were removed, and the supernatant was incubated with the primary antibodies for an additional 2 h at 4°C. Then, 100 μl of Protein ...
For co-IP, a 15 µL volume of HA antibody (3F10, Roche) was added to 1 mL of total protein extract and incubated at 4 °C for 4 h with gentle shaking before 20 μL of pre-rinsed protein G agarose beads (Millipore) was added. The mixture was incubated for another 3...
Protein G PLUS-AgaroseImmunoprecipitation Reagent: sc-2002Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.comPRODUCTProtein G PLUS is provided as an agarose conjugate for use in immunoprecipitationonly. The product is provided...
1) 取适量Protein A/G Agarose悬液,装入层析柱,用10倍柱体积的PBS(或者TBS,下同)洗涤平衡层析柱。 2) 含抗体的血清或其它体液高速离心后,将上清与等体积2×PBS缓冲液混合,调整pH以及离子浓度后,缓慢加入层析柱。 3) 用10倍柱体积以上的PBS洗涤,至流出液无蛋白检出。
The fusion proteins were purified using MBP agarose according to the manufacturer’s protocol. Apart from MBP-GR5 and empty MBP vectors, other constructs were treated and induced using a cell-free protein expression kit (Arbor) according to the manufacturer’s instructions. The recombinant proteins...
产品名称:CaM Kinase IIβ Protein, active, 10 µg 产品型号:14-718 Active, N-terminal 6His-tagged, recombinant, CaM Kinase IIβ, amino acids 1-315, for use in Kinase Assays. merckmillipore,默克密理博,14-718,CaM Kinase IIβ Protein, active, 10 µg ...
After activation for 48 h, 1x106 T cells were seeded into a 24-well plate and 8 μg/mL polybrene (EMO Millipore, Cat# TR-1003-G) was added. Lentivirus was added into the wells in MOI of 50, and the final volume of medium was 500 μL. After 12 h of infection, the media was...
Most were found to specifically co-IP on α-FLAG agarose with FL-SMCR8 but not empty vector. Approximately 1% of the input lysate (lanes 1, 2) and 30% of the immunoprecipitate (lanes 4–7) were loaded on gels. IP reactions were in the presence or absence of 50 μg/ml RNases....