(Novagen) NiNTAresin Lysecell Washwithbuffercontaining~10mMimidazole Eluteproteinwith300mMimidazole Imidazole,competitivebinder ofHistag Spinthelysateat15,000gfor30min LoadsupernatanttoNi-NTAcolumn GeneralprotocolforpurificationofHis-taggedproteins Furtherpurifybysizeexclusion Exampleofexpressionvector-GSTfusion ...
PROTEINEXPRESSIONANDPURIFICATION9,233–245(1997)ARTICLENO.PT960700OverexpressionandLarge-ScaleProductionofRecombinantL-Methionine-a-deamino-g-mercaptomethane-lyaseforNovelAnticancerTherapyYuyingTan,MingxuXu,XuezhongTan,XiuyingTan,XiaoenWang,YoshiroSaikawa,TakeshiNagahama,XinghuaSun,MartinLenz,andRobertM.HoffmanAnt...
The auto-induced pellets were then lysed and purified as described in the His-tagged purification protocol using here Cobalt-Buster Nickel Affinity gel (Amocol) instead of HIS-Buster Nickel Affinity gel. Library construction The E. coli CJ236 dut− ung− strain (TaKaRa, E4141S), which lac...
expressionacidic peptidetandem multimerEscherichia coliThe inositol hexaphosphate (IHP) method for purification of the erythrocyte membrane protein 4.1 yields the largest quantity of pure protein of any published protocol. However, protein 4.1 isolated by this method was found to bind to KI-stripped ...
Protein expression and purification A spacer was added at the C terminus of each designed sequence (‘GSWS’ for the sequences that have neither a Trp residue nor more than two Tyr residues and ‘GS’ for others) to separate the designed region from the C-terminal 6xHis-tag. Genes encoding...
Gg-laforin is more soluble and stable than human laforin in vitro, and possesses similar activity as a glucan phosphatase. Therefore, it can be used to model human laforin in structure-function studies. We have established a protocol for purifying recombinant Gg-laforin in sufficient quantity for...
Expression and purification of correctly folded proteins typically require screening of different parameters such as protein variants, solubility enhancing tags or expression hosts. Parallel vector series that cover all variations are available, but not without compromise. We have established a fast, effici...
The purification and refolding protocol described here may be generally applicable to bacterial expression of other members of this growing family of related small leucine-rich proteoglycan core proteins. 展开 关键词: Myc mutants nodulation Nod mutants Phaseolus vulgaris rhizobium VAM DOI: 10.1006/abio....
Protein expression and purification M.HpaII-His6, LacI-biotin, and LacI-His6 were expressed and purified as previously described (Duxin et al., 2014). Lysine methylation of M.HpaII was carried out as described (Larsen et al., 2018). Xenopus RTEL1 open reading frame with an N-terminal ...
Gibco Protein Expression Protocol Calculator Protein Expression Essentials Protein Expression Optimization Bacterial Protein Expression Mammalian Protein Expression Insect Protein Expression Yeast Protein Expression Algal Protein Expression Cell-Free Protein Expression Protein Expression Services Transient...