A membrane protein is a substance that's embedded in cell membranes. It performs specific tasks that are essential for the cell to...
Note the increase in the thickening of the dystrophin staining in the sarcolemma. Scale bars = 100 μm. B, Western blot of the indicated proteins in heart lysates from 3-month-old and, E, embryo (E14.5) conditional mutants (smShcA−) and controls. C, electron microscopic analysis of ...
maintain the ‘windows’ of immunodetection so that overlap between related moieties is minimized and diagnostic utility is maximized. If these maxims are heeded, the incidence of ‘aberrant’ keratin expression in mesenchymal neoplasia should be low when using routinely processed (paraffin-embedded) cl...
The plasma membrane consists of (a) a single layer of phospholipid molecules (b) a double layer of phospholipid molecules in which proteins are embedded (c) several layers of protein and carbohydrate molecules (d) a triple layer of phos...
Fill in the blank: ___ consist of bundles of protein filaments called myofilaments. Anatomy of a Muscle Fiber: Each cell in skeletal muscle tissue is known as a muscle fiber or muscle cell. A muscle fiber is a large cell consisting of many nuclei (multinucleate). The m...
These thin filaments are embedded into the cytoplasmic densities (dense bodies) and exhibit the same polarity relative to their attachment points. The thin filaments have their plus (+) ends at the dense body and their minus (−) ends extending into the cellular cytoplasm. Thus, although the...
The rate of net ATP-dependent transport (right, ▴) was calculated by subtracting transport in the presence of 5′-AMP as a blank from transport in the presence of ATP. Right inset: Immunodetection of MRP5 in the sarcolemma vesicles (40 μg of total protein) and V79/MRP5 membrane ...
Transmembrane proteins that make up enzymes in the plasma membranes are made up of hydrophilic and hydrophobic regions. Most amino acids embedded in the membrane are [{Blank}] , while most amino acids facing the extracellular fluid ...
1 DMD patients and mdx mice (the mouse model for DMD) have mutations in the gene encoding dystrophin. These mutations result in the absence of dystrophin, a 427 kDa cytoskeletal protein located under the sarcolemma of muscle fibers.2 Through the N-terminal region, dystrophin interacts with F...
Samples for immunofluorescence microscopy were embedded in fresh Tissue Tek OCT (Sakura Finetek, the Netherlands), immediately frozen in liquid nitrogen-cooled isopentane and stored at -80 °C until further analysis. Muscle samples for western blotting were immediately snap-frozen in liquid nitrogen ...