Two-dimensional gel electrophoresis All protein samples from non-shock and shock groups were assessed by 2-DE; a minimum of three samples were run per group to ensure reproducibility. In the first dimension of the 2-DE, protein samples (80 μg) were applied to immobilized pH gradient (IPG)...
Cytoscape “Results Panel” displays the results of FunMod analysis. When a particular pathway is selected, all the proteins on this pathway are selected in the network as yellow nodes. Using FunMod we identified 33 different biological pathways that are significantly enriched in the Budd–Chiari ...
Besides, the same molecular weight band is recognized in macrophage whole lysate by monoclonal anti-FMNL1. In addition, IgG4 of MN patients recognizes a faint band with the same molecular weight of actin. Nitro cellulose membrane were cut perpendicular to the electrophoresis migration front to ...
Proteins from the plasma membrane- and mitochondria-enriched fractions or cytosolic proteins isolated from left ventricles were run on 2D gel electrophoresis, scanned and quantified with specific software to reveal differentially expressed proteins. Results: Nine proteins were found to show markedly altered...
We then performed a proteolytic digestion of the SP–I3mEos complex, using either native aCt, aCt–cPA or B(aCt), and analysed the products of enzymatic fragmentation by gel electrophoresis (Fig. 4j and Supplementary Fig. 34). The data showed that, in all cases, the I3 portion of the ...
Denatured protein samples were separated by electrophoresis in 10% SDS-PAGE, transferred onto nitrocellulose membranes, blocked with 5% milk in PBS-tween (PBST), washed with PBST, and incubated with primary antibodies (described above) diluted in PBST overnight at 4°C. Primary antibodies were det...
(courtesy of Dr. Peter Davies). Samples were reduced in electrophoresis sample buffer containing 2.5% β-mercaptoethanol (v/v) at 60 °C for 15 min. Suppl. Figure1shows differences in tau forms contained in the TBS and SI fractions. PHF-1 positive signals appeared mostly as a smear of ...
The wide mass range (1—300 kDa), high accuracy, and sensitivity make it a superior method for analysis of all kinds of biomolecules including proteins, nucleic acids, and carbohydrates. In combination with 2D-gelelectrophoresis, MALDI-TOF-MS is particularly suitable for the identification of ...
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Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers J. Chromatogr. A, 1117 (2006), pp. 1-10 View PDFView articleGoogle Scholar [50] G. Perret, P. Santambien, E. Boschetti The quest for affinity chromatography ligands: are the molecular...