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In light of the broad applications of synthetic fusion proteins and the growing interest in their construction, providing a comprehensive review to summarize the commonly used design principles and construction strategies would be extremely useful. To this end, we focus on three distinct approaches, ta...
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51. In addition, the G251W mutation in WT TEM-1 had one of the lowest predicted fitnesses of any point mutation in the functional designs. The opt.b design had the lowest performance of any functional design
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Step 6: Review and collect the results of the NPCL nested-PCR primer design conducted by UPrimer. The final NPCL primers of the order Lepidoptera can be found in the folder "6.Designed_nested-PCR_primer_set_of_NPCLs_PIratio_3". The reference NPCL nucleotide and peptide sequences can ...
Yun Cheng (Conceptualization: Lead; Data curation: Lead; Formal analysis: Lead; Funding acquisition: Equal; Investigation: Lead; Methodology: Lead; Project administration: Equal; Resources: Supporting; Supervision: Supporting; Validation: Equal; Writing – original draft: Lead; Writing – review & edi...
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Nuclear-encoded mitochondrial proteins destined for the matrix have to be transported across two membranes. The TOM and TIM23 complexes facilitate the transport of precursor proteins with N-terminal targeting signals into the matrix. During transport, pr